检测胃癌患者外周血中K-ras和p53基因突变的临床意义

To detect the clinical significance of K-ras and p53 mutations in peripheral blood of patients with gastric cancer

目的分析胃癌患者外周血中K-ras和p53基因突变的临床价值。方法选取2013-02—2014-02我院收治的54例胃癌患者进行研究，采用PCR—SSCP技术对患者外周血中K—ras和p53基因突变情况进行测定并分析。结果54倒胃癌患者中K—Tas基因突变为7．41％，p53突变率为9．26％，总突变率为11．11％；有无肝转移患者外周血K—ras和p53基因突变阳性率差异有统计学意义，P〈0．05；分化程度与胃癌分期基因突变阳性率差异无统计学意义，P〉0．05。结论对胃癌患者外周血中K—ras和p53基因突变率进行分析，有利于帮助临床医师判断胃癌分期，为胃癌肝转移的预测、诊断提供科学的参考依据，值得临床推广应用。

OBJECTIVE To analyze the clinical value of K-ras and p53 mutations in the peripheral blood of patients with gastric cancer. METHODS Fifty-four cases of gastric cancer patients treated in our hospital from February 2013 to February 2014 were studied, and the K-ras and p53 mutations in peripheral blood of patients were determined and ana- lyzed. RESULTS In the 54 cases of gastric cancer, the mutation rate of K-ras gene was 9.26%, the rate of p53 mutation was 11.11%, and the total mutation rate was 7.41%. There was no significant difference in the positive rate of K-ras and p53 gene mutations （P〉0. 05） in patients with liver metastasis. CONCLUSIONS The mutation rate of K-ras and p53 in the peripheral blood of patients with gastric cancer was analyzed. It is helpful to help clinical doctors to determine the staging of gastric cancer, and to provide a scientific basis for the prediction and diagnosis of liver metastasis.