摘要
目的:探讨MGMT和hMLH1在弥漫性大B细胞淋巴瘤(DLBCL)免疫亚型组织中的表达及临床病理特征的关系。方法:采用逆转录聚合酶链反应(RT-PCR)检测100例初治DLBCL患者肿瘤组织中MGMT和hMLH1的表达情况,分析两者与DLBCL临床病理特征的关系。结果:MGMT及hMLH1的表达与临床分期、国际预后指数(IPI)、病理免疫亚型密切相关(均P<0.05)。MGMT在non-GCB亚型中的表达与生存率有关(P<0.05);hMLH1在GCB亚型及non-GCB亚型中的表达均与生存率有关(均P<0.05)。MGMT与hMLH1表达无明显相关关系(P>0.05)。结论:MGMT和hMLH1检测有助于判断DLBCL临床亚型和预后。
Objective:To investigate the expression of MGMT and hMLH1 in immunohistochemical classification of diffuse large B cell lymphoma(DLBCL)and its correlation with clinicopathological features.Methods:100cases of newly diagnosed patients with DLBCL tumor tissues were detected by RT-PCR assays for MGMT and hMLH1,and their correlation with clinical parameters were analyzed.Results:The expression of MGMT and hMLH1 were significantly correlated with the clinical stage,international prognostic index(IPI)and immunohistochemical classification of DLBCL.The expression of MGMT was related with survival rate in non-GCB subtype.The expression of hMLH1 was correlated with survival rate in GCB subtype and non-GCB subtype significantly(P〈0.05).There was no correlation between MGMT and hMLH1.Conclusion:Detection of MGMT and hMLH1 expressions may be helpful for determining the clinical subtypes,and could become prognostic factors for DLBCL.
出处
《广西医科大学学报》
CAS
2015年第4期556-559,共4页
Journal of Guangxi Medical University
基金
广西壮族自治区卫生厅重点课题资助项目(No.重2012096)
广西壮族自治区卫生厅自筹课题资助项目(No.Z2011222)
关键词
MGMT
HMLH1
弥漫性大B细胞淋巴瘤
免疫分型
逆转录聚合酶链反应
O6-methylguanine DNA methyltransferase
human MutL homolog 1
diffuse large B cell lym-phoma
immunohistochemical calssification
reverse transcriptase polymerase chain reaction