MGMT和hMLH1在弥漫性大B细胞淋巴瘤免疫亚型中的表达及临床意义

EXPRESSION AND THE CLINICAL SIGNIFICANCE OF MGMT,hMLH1 IN IMMUNOHISTOCHEMICAL CLASSIFICATION OF DIFFUSE LARGE B CELL LYMPHOMA

目的:探讨MGMT和hMLH1在弥漫性大B细胞淋巴瘤(DLBCL)免疫亚型组织中的表达及临床病理特征的关系。方法:采用逆转录聚合酶链反应(RT-PCR)检测100例初治DLBCL患者肿瘤组织中MGMT和hMLH1的表达情况,分析两者与DLBCL临床病理特征的关系。结果:MGMT及hMLH1的表达与临床分期、国际预后指数(IPI)、病理免疫亚型密切相关(均P<0.05)。MGMT在non-GCB亚型中的表达与生存率有关(P<0.05);hMLH1在GCB亚型及non-GCB亚型中的表达均与生存率有关(均P<0.05)。MGMT与hMLH1表达无明显相关关系(P>0.05)。结论:MGMT和hMLH1检测有助于判断DLBCL临床亚型和预后。

Objective：To investigate the expression of MGMT and hMLH1 in immunohistochemical classification of diffuse large B cell lymphoma（DLBCL）and its correlation with clinicopathological features.Methods：100cases of newly diagnosed patients with DLBCL tumor tissues were detected by RT-PCR assays for MGMT and hMLH1,and their correlation with clinical parameters were analyzed.Results：The expression of MGMT and hMLH1 were significantly correlated with the clinical stage,international prognostic index（IPI）and immunohistochemical classification of DLBCL.The expression of MGMT was related with survival rate in non-GCB subtype.The expression of hMLH1 was correlated with survival rate in GCB subtype and non-GCB subtype significantly（P〈0.05）.There was no correlation between MGMT and hMLH1.Conclusion：Detection of MGMT and hMLH1 expressions may be helpful for determining the clinical subtypes,and could become prognostic factors for DLBCL.