儿童血清25羟基维生素D水平及其基因多态性与哮喘的相关性研究

Study on the Correlation of 25-(OH)-VD and Vitamin D Receptor Gene Polymorphisms of Children and Asthma

目的通过对哮喘患儿血清中血清25羟基维生素D水平及维生素D受体基因多态性分析,拟探讨25-(OH)-VD在哮喘发病中的作用。方法本院门诊随诊的40例哮喘患儿为哮喘组,健康体检的儿童40例为对照组。采用酶联免疫吸附试验检测两组患儿血清25羟基维生素D水平,采用DNA测序技术检测两组患儿VDR基因位点rs2228570、rs7975232、rs11574114、rs1544410、rs731236、rs757343有无多态性存在。结果哮喘组儿童血清25-(OH)-VD水平均低于正常对照组,差异有统计学意义(t=7.498,P<0.001)。VDR基因序列rs7975232(ApaI)基因型GG、GT,rs731236(TaqI)基因型TT、TC,rs11574114基因型GG、GC、CC,rs1544410(Bsm I)基因型GG、GA,rs757343(Tru9I)基因型GG、GA、AA,在两组间基因型的分布有明显的差异(P<0.01或P<0.05)。结论哮喘患儿存在血清25-(OH)-VD不足或缺乏,VDR基因多态位点rs7975232、rs1544410、rs731236、rs11574114、rs757343基因型及等位基因分布在两组间存在差异,其多态性可能与哮喘发病相关。

Objective To explore the correlation of the 25-（OH）-VD and vitamin D receptor gene polymorphisms in children and asthma by detecting the level of 25-（OH）-VD and analyzing the gene polymorphisms of vitamin D receptor genes. Methods Forty children with asthma（clinical paracmasis） were enrolled as asthmatic group. Meanwhile, forty healthy children were selected as control group. Detecting the serum 25-（OH）-VD level in two groups children by the enzyme linked immunosorbent assay （ELISA）, meanwhile, examining the gene potymorphisms of vitamin D receptor gene rs2228570, rs7975232, rs1544410, rs731236, rs757343 by polymerase chain reaction （PCR）, gene clone and DNA sequencing. Results The levels of 25-（OH）-VD in serum in asthma children were obviously lower than those in control children（t=7. 498, P〈0. 001）;the distributions of the genetypes GG and GT of vitamin D receptor gene rs7975232 （ApaD ,the distributions of the genetypes TT and TC of rs731236 （TaqI）, the distributions of the genetypes GG,GC and CC of rs11574114, the distributions of the genetypes GG and GA of rs1544410 （Bsm I）, the distributions of the genetypes GG,GA and AA of rs757343（Tru9I） in two groups children were all obviously different（P〈0.01 or P〈 0.05）. Conclusion There were deficiency or lack of 25-（OH）-VD in serum in asthma children,the distributions of vitamin D genetypes and allele of rs7975232, rs1544410, rs731236, rs11574114 and rs757343 were obviously different, the polymorphisms may be correlated to the pathogenesis of asthma.