VEGF和MMP-2在胃癌中的表达及其在浸润和转移中的作用

The Expression of VEGF and MMP-2 in Gastric Carcinoma and Their Roles in the Invasion and Metastasis

目的 探讨血管内皮生长因子 (VEGF)和基质金属蛋白酶 -2 (MMP -2 )在胃癌发展、浸润和转移中的作用及二者相互关系。方法 应用免疫组织化学SP法检测 3 4例胃癌前病变和 68例胃癌组织中VEGF和MMP -2表达情况。结果 VEGF随胃癌的发展阳性率逐渐增高 (P <0 .0 5 ) ;侵及浆膜层组VEGF阳性率显著高于未侵及浆膜层组 (P <0 .0 0 5 ) ;淋巴结转移组显著高于无淋巴结转移组 (P <0 .0 1)。MMP -2随胃癌的发生、发展阳性率逐渐增高 (P <0 .0 5 ) ;侵及浆膜层组MMP -2表达率高于未侵及浆膜组 (P <0 .0 5 ) ;淋巴结转移组MMP -2表达率显著高于无淋巴结转移组 (P <0 .0 5 )。经统计学处理 ,VEGF和MMP -2表达关系密切 (P <0 .0 0 5 )。结论 VEGF和MMP -2均参与胃癌的发展、浸润和转移 。

Objective To evaluate the roles of VEGF and MMP-2 in the development,invasion and metastasis of gastric carcinoma as well as their interaction.Methods Streptacidin/Peroxidase(SP) technique was adopted to examine the expression of VEGF,MMP-2 in 34 cases of precancerous lesions and 68 cases of gastric carcinoma.Results With the development of gastric carcinoma,the positive rates of VEGF increased gradually( P <0.05).The positive rate of VEGF in the group with seroas infiltraiton was significantly higher than that in the group without serosa infiltration( P < 0.05). The positive rate of VEGF in the group with lymph node metastasis was higher( P <0.01) than that in the group without lymph node metastasis.With the incurrance and development of gastric carcinoma,the positive rates of MMP-2 increased gradually( P <0.05).The positive rate of MMP-2 in the group with serosa infiltration was higher than that in the group without serosa infiltration( P <0.05).The positive rate of MMP-2 in the group with lymph node metastasis was higher than that in the group without lymph node metastasis( P <0.05).By statistical analysis,there was close correlation between VEGF and MMP-2 expression( P <0.05).Conclusions Both VEGF and MMP-2 contribute to the development,invasion and metastasis of gastric carcinoma.They are indexes to know the biological behavior of and judge the prognosis of gastric carcinoma.