p53和Ki-67在苏丹红Ⅰ染毒小鼠肺组织中的表达

Expression of p53 and Ki-67 in lung tissue of mice exposed to Sudan Ⅰ

目的研究苏丹红Ⅰ对小鼠肺组织的损伤及肺组织p53和Ki-67表达的影响。方法昆明小鼠40只,随机分为4组:空白对照组,苏丹红Ⅰ低剂量(60 mg/kg)和苏丹红Ⅰ中剂量(120 mg/kg),苏丹红Ⅰ高剂量(240 mg/kg)组,10只/组。连续胃灌两周,于两周末处死,HE染色观察病理变化,免疫组化法检测肺组织p53和Ki-67表达。结果与对照组比较,实验组小鼠的肺泡隔增厚,且随着苏丹红Ⅰ浓度的增加,可见部分支气管充血和炎症细胞浸润;Ki-67在各组中表达差异有统计学意义(F=68.26,P<0.001),中浓度、高浓度组与对照组比较表达降低(均有P<0.05),p53在各组中表达差异有统计学意义(F=23.26,P<0.001),各浓度组与对照组比较表达增加(均有P<0.05)。结论苏丹红Ⅰ通过增加凋亡,抑制增殖来损伤小鼠肺组织。

Objective To study the damage and expression of p53 and Ki-67 in the lung tissues of mice by gastric garage of Sudan I. Methods Forty Kunming mice were randomly divided into 4 groups ： blank control group, low dose （60 mg/kg） , middle dose （ 120 mg/kg） and high dose （240 mg/kg） groups. Each group contained 10 mice. Gastric ga- vage was performed for two weeks, the pathological changes were observed by hematoxylin-eosin staining. The expression of 1o53 and Ki-67 in lung tissue, was detected by immunohistochemistry. Results With the ineresase of the concentration of Sudan I, part of bronchi became hyperemia infiltrated by inflammatory cell. The expression of Ki-67 was significantly differ- ent in each group （ F = 68. 26, P 〈 0. 001 ）. The expression of p53 was significantly different in each group （ F = 23.26, P 〈 0. 001 ）. Compared with that of the control group, the rates of Ki-67 positive cells were significantly lower in high/middle concentration groups （ all P 〈 0. 05 ）, the rates of p53 positive cells were significantly increased （ all P 〈 0. 05 ） in the three experimental groups. Conclusions Sudan I destroys lung tissues by increasing cell apoptosis and inhibiting cell prolifera- tion.