维生素B12对ROU家兔血清中IL-6、IL-8表达的影响

The influence of vitamin B12 with interleukin-6(IL-6)and interleukin-8(IL-8)levels oral ulcers in rabbit

目的:研究维生素B12对多发性口腔溃疡(ROU)家兔血清中白介素-6(IL-6)、白介素-8(IL-8)表达水平的影响。方法:将40只家兔随机分为4组,每组10只,随机选取10只设立空白对照组,其余30只用免疫法建立ROU家兔模型,然后随机分3组,分别为阳性对照组、左旋咪唑组及维生素B12组。观察口腔溃疡的变化,采用酶联免疫吸附法检测各组家兔灌胃结束后血清中IL-6、IL-8的含量。结果:阳性对照组与空白对照组比较,阳性对照组中IL-6、IL-8含量明显升高,差异具有统计学意义(P<0.01);左旋咪唑组和维生素B12组与阳性对照组比较,两实验组中的IL-6、IL-8水平明显低于阳性对照组,差异具有统计学意义(P<0.01);维生素B12组与左旋咪唑组相比,差异无统计学意义。结论:维生素B12通过补充微量元素,调节机体免疫功能,调控机体中细胞因子的表达,显著降低血清中IL-6、IL-8的高水平含量,这可能为其治疗ROU的作用机制之一。

Objective： To study the influence of vitamin B12 with interleukin-6 （IL-6） and interleukin-8 （IL-8） levels oral ulcers in rabbit. Method：40 rabbits were randomly divided into four groups（n=10）, 10 were randomly selected to set up the control group, and the remaining 30 made by immunization of rabbits with recurrent oral ulcers model [1] ,model after randomization the establishment of the positive control group, levamisole group and vitamin B12 group. Result： Observed the changes of mouth ulcers, in the use of IL-6 and IL-8 in serum after the enzyme-linked immunosorbent assay to detect the end of each group of rabbits intragastric administration. Results Compared the control group with the control group, P 〈0.01, the difference was statistically significant, ulcer group were significantly increased in the level of IL-6 and IL-8; levamisole group and vitamin B 12 positive group compared with the positive control group ulcer, P 〈0.01, the difference was statistically significant in both groups of IL-6 and IL-8 levels were significantly lower than the positive control group; levamisole group compared with vitamin B12 group,the difference was not statistically significant. Conclusion： Vitamin B12 through micronu- trient supplementation,regulate immune function, expression and regulation of cytokines in the body, significantly reduce IL- 6, IL-8 high-level content in serum, This may be one of the mechanisms of treatment for recurrent oral ulcers.