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淋巴细胞有丝分裂原制备法的改进及其在肿瘤患者中的应用

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摘要 目的 改进刺激淋巴细胞转化的方法和提取有丝分裂原条件使常规淋巴细胞转化率趋向稳定。方法 取各种原材料 :美洲商陆、刀豆、广州红豆、四季豆等 ,在不同的时间、温度下对淋巴细胞转化能力所产生差异 ,和采用正常与患者两者血样各自的空白与实验四联对照法措施 ,获得其中较理想条件。结果 对 2 5例正常人和 88例肿瘤患者 (其中食管癌 44例 )血样淋巴细胞转化率测试表明正常与病人间有明显差异 (P <0 0 0 1)。结论 采用四季豆 (郑州地豆 )定时、定量的有丝分裂原 ,测微计计量和四联检验等措施获得稳定的细胞转化率并证明肿瘤患者与正常人之间有显著差异 (P <0 0 0 1)
出处 《河南肿瘤学杂志》 2002年第4期283-284,共2页 Henan Journal of Oncology
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