摘要
选取抑癌基因和癌基因转录起始位点和转录终止位点上下2000bp,研究它们在癌细胞和正常细胞中甲基化分布差别,结果表明抑癌基因在癌细胞比正常细胞甲基化分布高,并且抑癌基因甲基化分布集中于转录起始位点前后1000bp,而原癌基因的甲基化分布要比抑癌基因的分布广.然后又对每个抑癌基因和原癌基因转录起始位点和转录终止位点的甲基化水平进行了研究,找出了在所选的癌细胞比正常细胞甲基化高的抑癌基因RUNX3,WT1,发现它们都是转录因子且生物学功能相似.
The difference of methylation between the tumor suppressor genes and the oncogenes flanking transcription start site （TSS） and transcription termination site （TTS） around 2000bp in cancer cells and normal cells is calculated. The results show that the distribution of methylation of tumor suppressor genes in cancer cells is higher than in normal cells, these genes mainly locate in 1000bp region flanking transcription start site, and the distribution of methylation of oncogenes has wider region than tumor suppressor genes. By studying the value of methylation for every gene flan- king the transcription start site and transcription termination site, there are two tumor suppressor genes,RUNX3 and WT1 which have the biggest difference of methylation in cancer cells and normal cells,and both of them are transcription factors and the thebiological function is similar.
出处
《内蒙古大学学报（自然科学版）》
CAS
北大核心
2015年第6期630-636,共7页
Journal of Inner Mongolia University：Natural Science Edition
基金
国家自然科学基金资助项目(No.31460234)
