前列消癥汤对前列腺癌PC-3干细胞增殖及表面标记的影响

The Effect of Qianliexiaozheng Decotion on PC-3 Prostate Cancer Stem Cells

目的:探讨前列消癥汤对前列腺癌PC-3干细胞增殖及表面标记的影响。方法:采用免疫磁珠分离法分离前列腺癌PC-3干细胞,提取前列腺癌干细胞,采用不同质量浓度(3 mg·L-1,10 mg·L-1,30 mg·L-1,100 mg·L-1)前列消癥汤、对照药物PC-SPES(30 mg·L-1)干预干细胞24 h,划痕实验测定前列腺癌PC-3干细胞迁移能力,流式细胞仪测定PC-3干细胞表面标记CD133、CD44,观察前列消癥汤对PC-3干细胞增殖及表面标记的影响。结果:免疫磁珠分离法分选获得高纯度的CD133+/CD44+PC-3前列腺癌干细胞;流式细胞仪检测CD133+和CD44+的阳性比例分别为97.12%和96.89%。前列消癥汤能抑制前列腺癌PC-3干细胞的迁移能力,并随着浓度的增加,其抑制迁移能力也随之增加。前列消癥汤能抑制前列腺癌PC-3干细胞标记CD133和CD44的表达,在一定范围内呈现浓度依赖性。结论:前列消癥汤具有抑制前列腺癌PC-3干细胞增殖及表面标记的作用。

Objective： To investigate the effect of QianL ieX iaoZ heng Decotion on PC-3 prostate cancer stem cell＇ s proliferation and its surface marker. Methods： Select the stem cells from PC-3 prostate cancer cells with Magnetic-Activated Cell Sorting method,and then observe the cells under different concentration of QianL ieX iaoZ heng Decotion（ 3 mg·L- 1,10 mg·L- 1,30 mg·L- 1,100 mg·L- 1）,being compared with the controlled drug PC-SPES（ 30 mg·L- 1） after 24 hours measuring PC-3 prostate cancer stem cells migration by scratch test and the surface marker CD133,CD44 of cells by flow cytometry to find out the effect of QianL ieX iaoZ heng Decotion on PC-3prostate cancer stem cells＇ proliferation and surface marker. Result： Prostate cancer stem cells were obtained by magnetic-activated cell sorting through the cell surface marker CD133,CD44,and the percentage of CD＋＋133and CD44 were 97. 12%,96. 89% respectively.QianL ieX iaoZ heng Decotion can inhibit the migration of PC-3 stem cell,and the inhibition effect become stronger with the increasing of concentration. The Decoction can inhibit the expression of PC-3 stem cell marker CD133,CD44,working in concentration dependent model in a certain range. Conclusion： QianL ieX iaoZ heng Decoction can inhibit the proliferation and surface marker of PC-3 to some extent.