原发性肝癌患者外周血辅助性T细胞17及白介素17水平的临床意义

Clinical significance of T helper 17 cells and related IL-17 level in patients with primary liver cancer

目的观察原发性肝癌(PHC)患者外周血中辅助性T细胞17(Th17)与细胞因子白介素17(IL-17)的分泌水平,探讨Th17细胞及IL-17与PHC病理发展的关系。方法选取PHC患者80例以同期40例健康体检健康者作为对照组,收集两组的外周血后采用流式细胞术检测Th17细胞亚群的比例,ELISA法检测血清中的IL-17水平,RT-PCR检测转录因子RORγT m RNA水平,并分析RORγT表达水平与PHC临床病理学特征的关系。结果 PHC患者外周血中Th17占CD4+T细胞比例为(4.09±0.33)%,显著高于对照组(1.38±0.27)%,差异有统计学意义(P<0.05);PHC患者外周血中IL-17水平为(57.73±6.21)pg/ml,显著高于对照组(33.98±4.47)pg/ml,差异有统计学意义(P<0.05);PHC患者外周血转录因子RORγT m RNA水平显著高于对照组,差异有统计学意义(P<0.001)。肝癌组织中RORγT m RNA的表达水平与肝癌组织Edmondson病理分级显著相关(r=0.685,P<0.05),且外周血中RORγT m RNA表达水平与HCC分化程度显著正相关(r=0.603,P<0.05)。结论 Th17及IL-17及转录因子RORγT m RNA水平在PHC患者外周血表达增加,且RORγT与PHC的病理分级程度明显相关。

【Objective】 To observe the T helper 17（Th17） cells and interleukin 17（IL-17） level in primary hepatocarcinoma（PHC）,and explore the relationship between Th17/IL-17 and pathological development of PHC.【Methods】 A total of 80 cases of PHC were collected as study group and 40 cases of healthy sunjects as control group.Th17 cells in peripheral blood were analyzed by flow cytometry.Serum IL-17 level was detected by ELISA,and the transcription factor ROR γ T m RNA level was detected by RT-PCR.The relationship between PHC ROR γ T and clinicopathological features was also analyzed.【Results】 The percentage of Th17 cells in peripheral blood of the PHC patients [（4.09 ± 0.33）%] was significantly higher than that in the controls [（1.38 ± 0.27）%],the difference was statistically significant（P〈0.05）.The IL-17 level in the PHC patients [（57.73 ± 6.21） pg/ml] was also significantly higher than that in the controls [（33.98 ± 4.47） pg/ml,P〈0.05].The level of ROR γ T m RNA in peripheral blood of the PHC patients was significantly higher than that of the controls（P〈0.001）.ROR γ T m RNA expression in the PHC tissue was significantly correlated with the Edmondson pathological grade（r = 0.685,P〈0.05）,and ROR γ T m RNA expression in peripheral blood was positively correlated with the degree of PHC differentiation（r = 0.603,P〈0.05）.【Conclusions】 Th17 cells,IL-17 and ROR γ T m RNA levels are increased in peripheral blood of PHC patients,and ROR γ T m RNA expression is significantly correlated with the pathological grade of PHC,suggesting ROR γ T as latent factor in PHC diagnosis.