小鼠70％肝脏切除模型的改良

Modifications of a 70 % partial hepatectomy mice model

目的建立一种简易、高效的小鼠70％肝切除模型，为研究肝脏再生的细胞和分子生物学机制及病理学意义提供技术平台。方法8～12周C57／BL6小鼠（20—25g）随机分为经典组（不阻断入肝血流组）和改良组（阻断入肝血流组）。分别采用两种方法建立小鼠70％肝切除模型。观察术后小鼠的生存率和肝再生情况。结果改良组存活率（97．3％）高于经典组（86．7％），术后出血、胆漏和腔静脉狭窄发生率显著低于经典组；术后肝再生情况两组无明显差异。结论通过结扎血管阻断入肝血流，然后行肝叶切除法建立小鼠70％肝脏切除模型，可准确量化肝脏切除程度。该法简便易行、成功率高、并发症少，为肝再生的机理研究提供了理想的动物模型。

Objective To establish an effective and convenient mice model of 70% partial hepatec- tomy, which could provide a basis for investigating the underlying cellular and molecular mechanisms and pathophysiological significance of liver regeneration. Methods 8 to 12 weeks old C57/BL6 mice （20 25 g） were randomly divided into two groups： classic group, liver resection was performed after ligation of hepatic lobe＇s pedicle; and modified group, hepatic branch inflow vessels were ligated before resecting the liver lobes. The two methods were used for establishing the 70% partial hepatectomy mice model, respec- tively. After surgery, the status of remnant liver regeneration and the mouse survival rate were investigated. Results The survival rate of the modified group （ 97.3% ） was superior to that of the classic group （86. 7% ）. The complications of postoperative bleeding, bile leakage and vena cava stenosis were significantly reduced in the modified group. However, there was no significant difference on liver regeneration indicated by the increased weight of remnant liver between the two groups. Conclusions We have successfully established an modified 70% partial hepatectomy model in mice by ligating hepatic branch inflow vessels be- fore liver resection. This method could precisely and conveniently quantify the liver resected volume, and gained increased survival rate but low complication incidence. It＇s an ideal animal model for conducting study on the liver regeneration.