轮叶党参总皂苷预处理减轻大鼠肝缺血再灌注损伤

Codonopsis lanceolata saponin preconditioning alleviates hepatic ischemia/reperfusion injury in rats

目的探讨轮叶党参总皂苷（CLS）对大鼠肝脏缺血一再灌注损伤（HIRI）的保护作用及其机制。方法将大鼠随机分为3组，即假手术组（SO）、HIRI组、CLS组，每组10只。采用Pringle法制备大鼠肝HIRI损伤模型。CLS组术前7d开始经口投予CLS200mg·kg-1·d-1，SO组和HIRI组给予水10ml·kg-1·d-1。采用比色法检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、超氧化物歧化酶（SOD）、丙二醛（MDA）水平以及肝组织SOD和MDA水平，ELISA法测定血清和肝组织中白细胞介素-18（IL一18）含量及血清肿瘤坏死因子（TNF—α）含量。结果HIRI组血清ALT、AST、MDA、IL-18、TNF-α及肝组织MDA、IL-18水平显著高于SO组（P〈0．05），HIRI组血清和肝组织SOD活性显著低于HIRI组（P〈0．05）；CLS组血清AIJT、AST、MDA、IL-18、TNF-α及肝组织MDA、IL-18水平显著低于HIRI组（P〈0．05），血清和肝组织SOD活性显著高于HIRI组（P〈0．05）。组织切片显示，HIRI组肝组织有明显的细胞坏死灶及大量炎性细胞浸润，CLS组病变明显轻于HIRI组。结论轮叶党参皂苷预处理对缺血再灌注肝脏具有明显的保护作用，其机制可能与抑制炎性因子及增强肝脏的抗氧化能力有关。

Objective To investigate the protective effects of Codonopsis lanceolata saponin （CLS） on hepatic ischemia-reperfusion injury （HIRI） and its mechanism in rats. Methods The rats were divided randomly into 3 groups：sham-operated （SO） group, HIRI group and CLS group. HIRI model was made by Pringle method. CLS group was pretreated with CLS （200 mg . kg-1 .d-1） by intragastric administration for 7 days before operation, while SO group and HIRI group were given with distilled water 10 ml . kg-1 . d-l for 7 days. ALT, AST, SOD, MDA in serum, and SOD and MDA in hepatic tissue were detected by colorimetric method. IL-18 in serum and hepatic tissue was detected by ELISA. Results The levels of serum ALT, AST, MDA, IL-18, TNF-α and tissue MDA, IL-18 in HIRI group were significantly higher than those in SO group and CLS group （ all P 〈 0.05 ）, respectively. The serum and hepatic tissue SOD activity in HIRI group was significantly lower than that in SO group and CLS group （ all P 〈 0.05 ）. Histological observation revealed that the obvious cell necrotic loci with a large number of inflammatory cells infiltration in the HIRI group, while the lesion was obviously alleviated in CLS group. Conclusions Codonopsis lanceolata saponin preconditioning alleviates hepatic ischemia/reperfusion injury. The mechanism may be correlated with inhibiting inflammatory factors and enhancing anti-oxidation.