癫痫患儿血清高迁移率族蛋白1及白细胞介素-6水平变化的意义

Changes of high mobility group protein box 1 and interleukin-6 in epileptic children and their significance

目的探讨高迁移率族蛋白1（HMGB1）、白细胞介素-6（IL-6）在癫痫患儿血清水平变化的意义。方法收集郑州大学第一附属医院6个月-12岁住院的癫痫患儿85例作为癫痫组，25例体检健康儿童作为健康对照组。其中癫痫组再分为癫痫频发组20例、癫痫持续状态组16例、非癫痫频发或持续状态组49例。采集癫痫发作后12h内空腹外周静脉血，离心后备用，批量操作。采用双抗体夹心酶联免疫吸附法检测85例癫痫患儿及25例健康对照组儿童血清HMGB1及IL-6水平，采用SPSS17．0软件对数据进行统计学分析。结果癫痫频发组、癫痫持续状态组、非癫痫频发或持续状态组及健康对照组血清中IL-6中位水平分别为14．559ng／L、14．752ng／L、11．981ng／L、8．056ng／L，HMGB1中位水平分别为8621．g／L、8987．g／L、7870ng／L、4221ng／L。癫痫患儿血清中HMGB1及IL-6水平显著高于健康对照组，差异有统计学意义（P均〈0．05），癫痫频发组、癫痫持续状态组IL-6、HMGB1水平显著高于非癫痫频发或持续状态组，差异有统计学意义（P均〈0．05），癫痫频发组和癫痫持续状态组IL-6、HMGB1水平比较差异无统计学意义（P均〉0．05）。IL-6水平与HMGB1呈正相关（r=0．760，P〈0．05）。结论癫痫发作可以诱发炎性因子HMGB1及IL-6高表达，给予HMGB1及IL-6抑制剂可能有利于癫痫的控制。

Objective To explore the changes of high mobility protein group box 1 （HMGB1） and interleukin -6 （ IL - 6） in epileptic patients and discuss the significance. Methods Eighty - five children aged 6 months to 12 years old with idiopathic generalized or partial epilepsy diagnosed in the First Affiliated Hospital of Zhengzhou University as the epileptic group,and it consisted of 20 cases of frequent epilepsy subgroup,16 cases of status epilepticus subgroup, and 49 cases of non frequent or status epilepticus subgroup. Twenty - five healthy children in outpatient department were selected as the healthy controls. Fasting veinal blood was obtained after seizure within 12 hours. The serum was collected and kept in the - 80 ℃ refrigerator. Enzyme linked immunosorbent assay was adopted to investigate the levels of HMGB1 and IL -6 in the serum. The data were analyzed by using SPSS 17.0 software. Results The levels of IL -6 in the serum of frequent epilepsy subgroup, status epilepticus subgroup,non frequent or status epilepticus subgroup and the healthy controls were 14. 559 ng/L, 14. 752 ng/L, 11. 981 ng/L, 8. 056 ng/L, respectively, and the levels of HMGB1 were 8 621 ng/L,8 987 ng/L,7 870 ng/L,4 221 ng/L,respectively. The levels of IL-6,HMGB1 of epileptic groups were higher than the healthy controls, especial the levels of IL - 6, HMGB1 of frequent epilepsy subgroup and the status epilepticus subgroup were higher than other in non frequent or status epilepticus subgroup, but the levels of IL - 6, HMGB1 between epilepsy subgroup and status epilepticus subgroup had no obvious difference（ P 〉 0. 05 ）. The level of IL - 6 had a positive correlation with level of HMGB1 （ r = 0. 760, P 〈 0. 05 ）. Conclusions It suggests that HMGB1 and the cytokine network may contribute to the generation of epilepsy in children. The inhibitor of HMGB1 and IL - 6 play a potential role in treatment of epilepsy.