沙棘总黄酮防紫外线损伤的实验研究

The effect of seabuckthorn flavonoids against ultraviolet injury

目的通过中波紫外线(UVB)辐射皮肤损伤模型来探讨沙棘总黄酮对皮肤的保护修复作用,为寻找新型抗紫外线的药物提供实验依据。方法通过UVB反复照射,构建UVB辐射小鼠皮肤损伤模型,观察各组实验动物皮肤形态学变化、皮肤中超氧化物歧化酶(SOD)活性、皮肤内脂类受过氧化损伤的程度即丙二醛(MDA)的量和胶原蛋白量的变化程度。结果模型组较正常组皮肤形态学变化明显,表皮层增加,真皮层增厚,UVB辐射皮肤损伤模型构建成功;模型组较正常组SOD活性和MDA的量分别是极显著降低和升高(P<0.01),而阳性对照组和沙棘总黄酮给药组较模型组SOD的活性和MDA的量分别是极显著升高和降低(P<0.01);模型组较正常组胶原蛋白量显著降低(P<0.05),阳性对照组和沙棘总黄酮给药组胶原蛋白量较高(P<0.01),但阳性对照组出现了增生性瘢痕。结论沙棘总黄酮能有效防止UVB反复照射对皮肤造成的损伤,提高皮肤组织中SOD活性和胶原蛋白量,抑制MDA的量升高和瘢痕增生,并对UVB导致小鼠皮肤光老化有拮抗作用。

Objective Through the UVB rays radiation skin injury model,the study investigated the effect of seabuckthorn flavonoids on the protection and restoration of the skin,in order to provide experimental basis of looking for the new drugs against ultraviolet injury.Methods By UVB radiating repeatedly,the study built UVB radiation skin injury model in mice,to observed the morphological changes of the skin in every groups of experimental animal,the super oxide dismutase（SOD）activity of the skin and the peroxidation damage degree of the skin lipid,which is also the content changes of malondialdehyde（MDA）and collagen.Results The change of model group whose epidermis layer increased and the dermis thickened was more obvious than the normal group,so the UVB radiation skin injury model was built successfully.The model group compared with the normal group,its SOD activity was significantly lower and its MDA content was significantly higher（P〈0.01）.However,the SOD activities of positive control group and being fed with seabuckthorn flavonoids group were significantly higher and their MDA contents were significantly lower（P〈0.01）.The model group compared with the normal group,its collagen content was significantly lower（P〈0.05）,and the collagen contents of positive control group and being fed with seabuckthorn flavonoids group were higher,whose difference was extremely significant（P〈0.01）.However,the positive control group had hyperplastic scar.Conclusion Seabuckthorn flavonoids had the following effects：preventing the UVB repeating radiation damage to the skin,improving the SOD activity of skin tissue and the collagen content,inhibiting the increase of MDA content and scar hyperplasia,and having antagonism effect of UVB causing mice skin photoaging.