摘要
目的采用MALDI-TOF质谱分析方法鉴定院内感染致病菌。方法同步使用VITEK-2和MALDI-TOF质谱鉴定院内感染致病菌。从检测时间、鉴定率及检出数量进行两种方法的对比。将微生物大分子指纹图谱进行共性峰比对计算,获得院内感染致病菌株间同源性结构关系的差异。结果共检出31株大肠埃希氏菌、28株肺炎克雷伯氏菌及解鸟苷酸拉乌尔菌等罕见致病菌9株。MALDI-TOF质谱检测能够在1h内检出所有的致病菌,且鉴定率和检出数量高于VITEK-2。结论致病菌的院内感染呈现科室为中心点源传播的模式。MALDI-TOF质谱检测速度快、准确率高,能够进行临床耐药菌的聚类分析,有望成为致病菌鉴定和临床耐药菌风险监测工作的主要技术力量。
Objective Pathogens from the nosocomial infection have been analyzed by MALDI-TOF microbial identification system,to evaluate mass spectrometry analysis advantage and explore the mass spectrometry method.Methods The pathogens have been analyzed by MALDI-TOF microbial identification system,by compared with the VITEK-2compact detection in the testing time,detection rate and the amounts of identified strains.The homology differences have been analyzed by comparison calculation of common peaks from the fingerprint spectrums.Results Thirty-one Escherichia coli strains,28 Klebsiella pneumonia strains and 9unusual pathogen strains have been identified by MALDI-TOF MS for only 1hours.It has more advantages than VITEK-2in the testing time and other aspects.Conclusion Nosocomial infection of pathogen shows a point source propagation mode centering on the department.MALDI-TOF mass spectrometry is able to rapidly and correctly identify the pathogen.MALDI-TOF microbial identification system is expected to be the major detecting technique in the field of the pathogen monitor and resistance monitoring analysis.
出处
《重庆医学》
CAS
北大核心
2015年第33期4665-4670,共6页
Chongqing medicine
基金
市级科技局基金项目(CJ201130012)
