固本防哮饮对支气管哮喘模型小鼠肺组织炎症因子及STAT1蛋白表达的影响

The Effect of Guben Fangxiao Yin(固本防哮饮) on the Expression of Inflammatory Factor and STAT1 Protein in Lung Tissue of Bronchial Asthma Mouse Model

目的探讨固本防哮饮防治支气管哮喘的可能作用机制。方法将70只BALB/c小鼠随机分为正常组、模型组、孟鲁司特钠组和固本防哮饮低、中、高剂量组及固本防哮饮预防组,每组10只。除正常组外,其余各组小鼠采用卵蛋白致敏和激发联合呼吸道合胞病毒感染的方法,建立支气管哮喘模型。孟鲁司特钠组给予孟鲁司特钠片1.5 mg/(kg·d),固本防哮饮低、中、高剂量组分别给予固本防哮饮13.5、27.0、54.0 g/(kg·d),以上各组均于造模第56天开始灌胃给予相应药物,每天1次,连续28天。固本防哮饮预防组给予固本防哮饮13.5 g/(kg·d),于造模第1天开始灌胃,连续84天。取各组小鼠右肺中叶进行HE染色,检测肺组织白细胞介素6(IL-6)、白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)mRNA的表达及STAT1蛋白表达。结果 HE染色结果显示,模型组可见肺小叶正常结构破坏,肺泡壁明显增厚、充血、水肿及炎性细胞浸润,气道壁增厚,管腔狭窄;各药物组小鼠肺组织仅有轻度的炎性病变,较模型组明显减轻。与正常组比较,模型组小鼠肺组织STAT1蛋白及IL-6、IL-8、TNF-αmRNA表达水平显著升高(P<0.05);与模型组比较,孟鲁司特钠组和固本防哮饮低、中、高剂量组及预防组小鼠肺组织中STAT1蛋白、IL-6、IL-8 mRNA表达明显降低(P<0.05),孟鲁司特钠组和固本防哮饮中、高剂量组及预防组小鼠肺组织中TNF-αmRNA表达明显降低(P<0.01)。结论固本防哮饮防治支气管哮喘可能与抑制肺组织中STAT1蛋白的活化,降低IL-6、IL-8、TNF-αmRNA的表达有关。

Objective To research the possible mechanism of Guben Fangxiao Yin in preventing and treating bronchial asthma. Methods Randomly divided 70 BALB / c mice into normal group,model group,Montelukast group,Guben Fangxiao Yin low,middle,high dose group,and the Guben Fangxiao Yin prevention group,with 10 in each. Mice in other groups except the normal group adopted ovum albumin sensitization and stimulation with respiratory syncytial virus infections method to established bronchial asthma model. The Montelukast group was given Montelukast Sodium Tablets 1. 5 mg /（ kg·d）. The Guben Fangxiao Yin low,middle,high dose group were respectively given Guben Fangxiao Yin 13. 5,27. 0,54. 0 g /（ kg·d）. Above groups were all given corresponding medicine from the 56 th day after starting to make model,once per day,for 28 days continuously. The Guben Fangxiao Yin prevention group was given Guben Fangxiao Yin 13. 5 g /（ kg·d）. Began to gavage from the first day of modelling,for 84 days.Used the middle lobe in the mice ＇s right lungs in each group for HE dye. Examined the expression of lung tissue interleukin6（ IL-6）,interleukin8（ IL-8）,tumor necrosis factor α（ TNF-α） mRNA and the expression of STAT1 protein. Results HE dye results showed that normal structure of pulmonary lobule in the model group broke,the alveolar wall remarkably thickened,with hyperemia,edema and inflammatory cell infiltration,the airway wall thickened and lumen narrowed. Lung tissue of the mice in each medical groups only had slight inflammation lesion,remarkably decreasing compared with that of the model group. Compared with the normal group,the expression level of STAT1 protein and IL-6,IL-8,TNF-α mRNA of mouse lung tissue in the model group remarkably increased（ P〈 0. 05）; Compared with the model group,the expression of STAT1 protein and IL-6,IL-8,TNF-α mRNA of mice lung tissue in the Montelukast group,the Guben Fangxiao Yin low,middle,high dose group,and the Guben Fangxiao Yin prevention group remarkably decreased（ P〈 0. 05）; The expression of TNF-α mRNA of mice lung tissue in the Montelukast group,the Guben Fangxiao Yin middle,high dose group,and the Guben Fangxiao Yin prevention group remarkably decreased（ P〈 0. 01）. Conclusion Guben Fangxiao Yin could prevent and treat bronchial asthma,which may be related with inhibiting activating STAT1 protein in lung tissue and decreasing the expression of IL-6,IL-8,and TNF-α mRNA.