一种20%氯虫苯甲酰胺悬浮剂的液相色谱检测方法研究

Research on Chromatography Detection Method of 20% Chlorantraniliprole Suspension Liquid

为研究筛选出一种高效液相色谱技术检测20%氯虫苯甲酰胺悬浮剂的最佳方法,首先通过液相色谱检测不同浓度的标准品建立标准曲线(y=2.9×105x-1.8×106,R2=0.9999),然后通过对5种不同提取剂(甲醇、乙醇、乙腈、苯酮、二氯甲烷)的提取样品后液相色谱结果进行比较研究,筛选出其中较好的提取试剂-二氯甲烷,并且其最佳浓度为55%(20%氯虫苯甲酰胺悬浮剂-康宽的检出浓度为931.4μg/m L)。研究建立了一种氯虫苯甲酰胺最佳液相色谱方法(在常规的液相色谱条件下,以55%的二氯甲烷提取),为氯虫苯甲酰胺后续的残留以及消解等方面研究提供了参考。

This paper aims to find the best way to detect 20% chlorantraniliprole suspension liquid by usinghigh performance liquid chromatography（HPLC）. Firstly, the standard curve was built through detectingdifferent dosages of the standard sample method by HPLC. Secondly, the research showed that HPLC result ofdichloromethane was the best among 5 different extracting materials（solvent methanol, ethanol, acetonitrile,acetone and dichloromethane） and the best dosage for extracting was 55%. It was found that the best HPLC wayfor detecting 20% chlorantraniliprole suspension liquid was using 55% dichloromethane to extract thechlorantraniliprole（detectable concentration of 20% chlorantraniliprole SC- CORAGEN was 931.4 μg/m L）.The research established the best HPLC way to detect chlorantraniliprole and could provide references forfurther study on the residue and elimination of chlorantraniliprole.