摘要
为研究筛选出一种高效液相色谱技术检测20%氯虫苯甲酰胺悬浮剂的最佳方法,首先通过液相色谱检测不同浓度的标准品建立标准曲线(y=2.9×105x-1.8×106,R2=0.9999),然后通过对5种不同提取剂(甲醇、乙醇、乙腈、苯酮、二氯甲烷)的提取样品后液相色谱结果进行比较研究,筛选出其中较好的提取试剂-二氯甲烷,并且其最佳浓度为55%(20%氯虫苯甲酰胺悬浮剂-康宽的检出浓度为931.4μg/m L)。研究建立了一种氯虫苯甲酰胺最佳液相色谱方法(在常规的液相色谱条件下,以55%的二氯甲烷提取),为氯虫苯甲酰胺后续的残留以及消解等方面研究提供了参考。
This paper aims to find the best way to detect 20% chlorantraniliprole suspension liquid by usinghigh performance liquid chromatography(HPLC). Firstly, the standard curve was built through detectingdifferent dosages of the standard sample method by HPLC. Secondly, the research showed that HPLC result ofdichloromethane was the best among 5 different extracting materials(solvent methanol, ethanol, acetonitrile,acetone and dichloromethane) and the best dosage for extracting was 55%. It was found that the best HPLC wayfor detecting 20% chlorantraniliprole suspension liquid was using 55% dichloromethane to extract thechlorantraniliprole(detectable concentration of 20% chlorantraniliprole SC- CORAGEN was 931.4 μg/m L).The research established the best HPLC way to detect chlorantraniliprole and could provide references forfurther study on the residue and elimination of chlorantraniliprole.
出处
《中国农学通报》
2015年第31期213-216,共4页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金资助项目"p66shc-ROS轴介导猪早期胚胎体外发育阻滞机理的研究"(31301978)
湖南省教育厅科学研究青年项目"猪胚胎体外生产技术体系的研究与应用称"(13B056)
湖南省娄底市科技计划项目"猪冻精人工授精技术的研究与应用"[娄財企指2014(618)号文件]
湖南人文科技学院英才支持计划
湖南人文科技学院高层次人才科研启动基金