连作大豆苗期根际土壤根腐病病原真菌菌群结构分析

Analysis of Community Structure of Root Rot Pathogenic Fungi in Seedling Stage of Soybean Continuous Cropping

连作障碍是限制黑龙江地区大豆产质量提高的主要因素。本项研究的主要目的是探究连作大豆根腐病发病期病原真菌菌群结构,为生物防治大豆根腐病奠定基础。采用PCR-DGGE技术,分析不同连作年限苗期大豆土壤中的3个大豆品种根系以及根际土壤根腐病病原真菌菌群结构。试验结果表明:随着连作年限的延长,3个大豆品种苗期根系以及根际土壤根腐病病原真菌丰度值以及多样性指数随之增加;并且不同大豆品种间根腐病病原真菌菌群结构也存在差异;聚类分析结果验证了连作年限、大豆品种均对根腐病病原真菌菌群结构产生影响。

Continuous cropping obstacle is the main factor that restricts the improvement of soybean quality and yield in Heilongjiang region. The aim of this study was to investigate the community structure of root rot pathogenic fungi in the continuous cropping of soybean which could lay a foundation for bio-control of the soybean root rot. In this study, the community structure of root rot pathogen in the seedling stage of root and soil samples of three soybean varieties planted in different duration of continuous cropping soil was analyzed by PCR-DGGE technology. The result showed that along with the extension of continuous cropping duration, the index of abundance and diversity of the three soybean varieties of root rot pathogen was increased in the seedling stage of root and soil samples; furthermore, the community structure of root rot pathogen was different among the soybean varieties. The result of cluster analysis verified that both the duration of continuous cropping and soybean variety had an effect on the community structure of root rot pathogen.