气相色谱法测定培化磷脂酰乙醇胺中三氯甲烷残留量

Determination of Residual Chloroform in Phosphatidylethanolamine Pegol by GC

目的：建立培化磷脂酰乙醇胺中三氯甲烷残留量测定方法。方法：采用毛细管气相色谱法,色谱柱为DB-624;电子捕获检测器,柱温采用程序升温：起始温度为100℃,以50℃/min升至240℃,保持5 min。结果：在该色谱条件下,在0.04~7.1μg·m L-1范围内线性关系良好,相关系数为0.9993,平均回收率为100.3%,RSD为0.98%（n=9）;检测限为10 ng·m L-1。结论：该毛细管气相色谱法灵敏、准确、可靠,适用于本品中有机溶剂残留量测定。

Objective： A GC method was established for determination of residual chloroform in phosphatidylethanolamine pegol. Method： Using a DB-624 capillary column, with ECD as detector and program temperature. The initial temperature was kept at 100℃, then the temperature was raised to 240℃ at the rate of 50 ℃.min4 and kept for 5 minutes. Results： The calibration curves of chloroform showed good linearity in the range of 0.04-7.1 ktg.mLl （ r=0. 9993）. The average recovery was 100.3 % with RSD 0.98 % （n=9）. The detection limit was 10 ng·mL^-l. Conclusion： The method is accurate sensitive, simple and reliable, it is suitable for measurement of residual chloroform in the phosphatidylethanolamine pegol.