氯喹对MT01促进大鼠骨髓间充质干细胞增殖作用的影响

Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01

目的:通过检测氯喹(chloroquine,CQ)对MT01促进Wistar大鼠骨髓间充质干细胞(BMSCs)增殖的影响,证实MT01可能经由内吞小体对BMSCs细胞生物学性能产生影响。方法:不同工作浓度的CQ与第3代Wistar大鼠BMSCs共培养24、48、72、96h,检测CQ对BMSCs增殖影响的量效和时效关系;在此基础上,加入工作浓度为1mg/L的MT01,MTT法检测不同浓度、不同时间点CQ对MT01作用下BMSCs增殖的影响。结果:适当浓度CQ(4mg/L)具有促进BMSCs增殖的作用;48hCQ刺激BMSCs增殖最明显;低浓度CQ(1mg/L、2mg/L)对MT01促进BMSCs增殖具有抑制作用。结论:CQ对MT01促进BMSCs增殖具有抑制作用,提示MT01可能通过进入BMSCs内吞小体中发挥作用。

Objective：To confirm that MT01 may influence the biological properties of bone marrow mesenchymal stem cells（BMSCs）via endosome by detecting the effect of chloroquine（CQ）on the promotion of Wistar rat BMSCs proliferation by MT01.Methods：The third generation of Wistar rat BMSCs were cultured with different working concentrations of CQ for 24,48,72 and 96hours,in order to detect the effect of CQ on BMSCs proliferation in dose-response relationship and aging relationship.Then,MTT assay was employed to detect the influence of CQ on MT01-stimulated BMSCs proliferation at different concentrations and different time points by adding the 1mg/L working concentration of MT01.Results：Appropriate concentration of CQ（4mg//L）could promote BMSCs proliferation.CQ exhibited the strongest promotion to BMSCs proliferation after 48 hours.Low concentrations of CQ（1mg/L and 2mg/L）inhibited the MT01-mediated promotion of BMSCs proliferation.Conclusion：CQ has an inhibitory effect on the MT01-mediated promotion of BMSCs proliferation,suggesting that MT01 may play a role by entering the endosome in BMSCs.