牙齿煅烧颗粒结合富血小板纤维蛋白修复骨缺损的实验研究

Bone Regeneration Effects of Tooth Ash in Conjunction with Platelet-rich Fibrin in an Animal Model

目的:通过动物实验评价富血小板纤维蛋白(Platelet-Rich Fibrin,PRF)促进牙齿煅烧颗粒修复骨缺损的早期效果。方法:6月龄新西兰兔10只,于颅顶部制备4个骨缺损,每个骨缺损作为1个观察组。A组为空白对照,不填材料;B组为对照组,填入牙齿煅烧颗粒;C组为实验组,填入牙齿煅烧颗粒与PRF混合物;D组为阳性对照组,填入牙齿煅烧颗粒与骨髓混合物。术后6周取材,通过大体观察、X线检查和组织学分析骨缺损的修复效果。结果:各组骨缺损内均未见炎症反应,新骨生成率分别为:A组(7.5±2.2)%;B组(17.6±2.3)%;C组(21.0±4.7)%;D组(23.4%±3.2)%。统计学分析发现,B、C、D组的新骨生成率显著高于A组(P<0.05)。C组和D组的新骨生成率显著高于B组(P<0.05)。C组和D组间的新骨生成率没有显著性差异。结论:牙齿煅烧颗粒具有良好生物相容性和骨传导性,与PRF混合后可以显著加快新骨再生的速度,促进骨缺损的修复。

Objective：To assess the initial effects of tooth ash in conjunction with platelet-rich fibrin（PRF）on reconstruction of bone defect.Methods：Ten white rabbits were selected to create an animal model with four defects on the skull.Defects in group A was used as vacant control,tooth ash particles were used solely in group B,tooth ash mixed with PRF were used in group C,and the defects in group D were filled with tooth ash combined with bone marrow.The animals were sacrificed 6weeks later,bone samples were harvested for histological observation and statistical analysis.Results：The percentage of new bone formed in each group was 7.46%±2.21%,17.61%±2.29%,21.00%±4.73%,and 23.42%±3.22%,respectively.Group C and D showed a statistically greater amount of new bone formation compared with group A and B（P〈0.05）.No significant differences were found between group C and D.Conclusion：Teeth ash particle is a type of bone graft material with favorable biocompatibility and osteoconductivity.After in conjunction with PRF,it can facilitate the bone healing process in early stage.