不同处理方式对毛竹篼纤维素及木质素酶活的影响

Effects of Different Treatments on Cellulase and Lignin Enzyme Activity in Bamboo Stump

对新砍伐的毛竹篼进行促腐剂、机械、覆盖方式等处理,得出降解过程中滤纸酶活、棉花酶活、CMC酶活最高值分别为154.24×103、368.88×103、57.85×103U/kg,最低值分别为56.65×103、70.34×103、28.76×103U/kg;木质素过氧化物酶活、漆酶酶活、锰过氧化物酶活最高值分别为0.13×103、6.07×103、159.07×103U/kg,最低值分别为0.001×103、0.200×103、0.760×103U/kg。毛竹篼降解过程中棉花酶活>滤纸酶活>CMC酶活,锰过氧化物酶活>漆酶酶活>木质素过氧化物酶活;毛竹篼不同部位的纤维素酶活和木质素酶活是复杂多变的。总CMC酶活与总滤纸酶活存在显著正相关,覆盖处理对总木质素酶活的影响最大。

Through promote corruption reagents,mechanical treatment,covering manner with the newly cutting bamboo stump,the results showed that in the degradation process filter paper enzyme,the cotton cellulose enzyme and CMC enzyme activity supreme values was 154.24 ×10^3 ,368.88 ×10^3 ,57.85 ×10^3 U /kg,respectively,and minimum values was 56.65 ×10^3 ,70.34 ×10^3 ,28.76 ×10^3 U /kg,respectively.Lignin peroxidase enzyme,lacca-se and manganese peroxidase enzyme activity supreme values was 0.13 ×10^3 ,6.07 ×10^3 ,159.07 ×10^3 U /kg,re-spectively,and minimum values was 0.001 ×10^3 ,0.200 ×10^3 ,0.760 ×10^3 U /kg,respectively.In the degradation process of bamboo stump,the whole cellulase activity in order was cotton cellulose enzyme,filter paper enzyme and CMC enzyme activity;the lignin enzymes activity in order was manganese peroxidase,laccase and lignin peroxidase enzyme activity.While the different parts of bamboo stump of the cellulose activity and lignin enzyme activity were complex and changeable.Total CMC enzyme activity and total filter paper enzyme activity were significantly positive correlation.The influence of covering treatments on total lignin enzyme activity was largest.