超高效液相色谱-四极杆-静电场轨道阱质谱法分析不同炮制红参的化学成分差异

Analysis of Chemical Differences in Red Ginseng by UPLC-Q-Orbitrap Mass Spectrometry

采用超高效液相色谱-四极杆-静电场轨道阱质谱法(UPLC-Q-Orbitrap MS)对蒸制红参、醋制红参和烘干红参中的人参皂苷类成分进行分析研究。基于三醇型皂苷Re、Rg1、Rg2、Rh1标准品和二醇型皂苷Rb1、Rb2、Rc、Rd、Rg3、Rh2标准品的加热降解规律和质谱特征,鉴定出不同人参炮制品中的32个人参皂苷。其中,人参皂苷mRb1、Rh1、F1、20(R)-Rh1、Rg5和Rs5仅在醋制红参中检出,notoR1仅在蒸制红参中检出。该方法明确了3种人参炮制品的化学成分差异,可为指导人参不同炮制品的分类应用提供技术支撑。

Red ginseng is processed by steaming the fresh ginseng.The decay reaction and hydrolysis reaction were occurred to result in the kinds and contents of ginsenosides to change when the process of fresh ginseng.The purpose of the study is to evaluate the chemical transformation of steamed red ginseng,vinegared red ginseng and dried red ginseng by ultra high performance liquid chromatography coupled with quadrupoleorbitrap mass spectrometry（UPLC-Q-Orbitrap MS）.Based on the heat degradation passways and mass spectra data of panaxatriol saponins（Re,Rg1,Rg2,Rh1）and protopanaxodiol saponins（Rb1,Rb2,Rc,Rd,Rg3,Rh2）,32 kinds of ginsenosideswere identified in different processed red ginseng.Ginsenosides mRb1,Rh1,F1,20（R）-Rh1,Rg5 and Rs5were only found in red ginseng processed by vinegar,which notoR1 is only detected in streamed red ginseng.Ginsenosides mRb1 exist in fresh ginseng and white ginseng,which is easy to decompose,can be found in red ginseng processed by vinegar,but it was detected in steamed red ginseng and dried red ginseng.The results show that C-20 is easy to lose glucosyl in MS/MS,and which needs lower energy and can be used for reference to distinguish isomers.The acidic condition with stable function for methylmalonyl ginsenosides,which has the ability to promote the generation of rare ginsenosides at the same time.The application identified the chemical composition differences of 3kinds of ginseng products,which would guide the classification of the different processed red ginseng.The three kinds processed ginseng products would like different clinic application because of different chemical composition.The method is rapid and easy,which is suitable for natural products analysis and application.