不同采血管在不同存放条件下对循环酶法同型半胱氨酸测定结果的影响

Influence of different blood collection tubes and different storage conditions on results of homocysteine detection by enzymatic cycle method

目的探讨不同的血样采集管,不同存放时间及不同存放温度对同型半胱氨酸（Hcy）测定结果的影响。方法选取32例健康体检者,分别用普通真空采血管、促凝真空采血管、肝素锂抗凝真空采血管、EDTA-K2抗凝真空采血管各抽取两管静脉血,将同一受检者的相同采血管分别分入室温组和冷藏组,编号并于室温放置0.5h离心,离心后立即将冷藏组置于冰箱,并检测室温组Hcy浓度;同时立刻吸取每例受检者普通真空采血管及肝素锂抗凝真空采血管血清及血浆200μL于1mL EP管并置于2-8℃冰箱冷藏保存,为分离组。在离心后3、6、9h测定室温组及冷藏组Hcy浓度,测定完成后选取冷藏组普通真空采血管及肝素锂抗凝真空采血管继续在冰箱中放置为未分离组,并在24、48h测定分离组与未分离组Hcy浓度。结果 4种采血管标本的Hcy即刻值血清标本高于血浆标本（P〈0.05）。同组相同采血管放置3、6、9h与各自即刻值比较差异无统计学意义（P〉0.05）。在相同测定时间比较同种采血管室温组与冷藏组的Hcy浓度结果,9h测定值比较差异有统计学意义（P〈0.05）。不及时分离血细胞层,普通真空采血管血清和肝素锂抗凝真空采血管血浆中Hcy浓度在2-8℃下,48h结果与即刻值比较差异均有统计学意义（P〈0.05）。结论不同的血样采集管,不同存放时间及不同存放温度都会影响循环酶法Hcy的测定结果。

Objective To study the influence of different blood sample collection tubes,different storage time and different storage temperatures on the results of homocysteine（Hcy）detection by enzymatic cycle assay.Methods The venous blood sample was collected from 32 individuals undergoing the healthy physical examination.The general vacuum blood collection tubes,procoagulant vacuum blood collection tubes,lithium heparin anticoagulation vacuum blood collection tubes and EDTA-K2 anticoagulation vacuum blood collection tubes were respectively adopted to collect 2tubes of venous blood for each kind of tube.The same blood tubes from the same person were divided into the room temperature group and the cold storage group respectively,centrifuged after giving serial number and storing for 0.5hat the room temperature,Hcy in the room temperature group was detected at once,while the cold storage group was stored in the fridge;meanwhile 200μL of serum and plasma were extracted from the general tube and lithium heparin anticoagulation tube and placed into 1 mL EP tube for storage in fridge at 2-8 ℃（separation group）.The Hcy levels in the room temperature group and the cold storage group were detected at 3,6,9hafter centrifugation.After detection,the general tube and lithium heparin anticoagulation tube in the cold storage group were continuously placed in fridge（non-separation group）.The Hcy levels in the separation group and the non-separation group were detected at 24,48 h.Results The serum Hcy instant values of 4kinds of blood collection tube for serum specimen were significantly higher than those for plasma specimen（P〈0.05）.The Hcy levels had no statistically significant differences between the placing for 3,6,9hby the same collection tube in the same group and the instant values（P〈0.05）.In the comparison of the Hcy detection results of the same blood collection tube at the same detection time between the room temperature group and the cold storage group,the detection value at 9hhad statistical difference（P〈0.05）.The Hcy levels at 48 hunder 2-8 ℃in the general tube serum and lithium heparin anticoagulation tube plasma without timely separating blood cell layers had statistical difference compared with the results detected at48h（P〈0.05）.Conclusion Different blood collection tubes,different storage time and different storage temperatures will affect the detection results of Hcy by enzymatic cycle assay.