基于DNA条形码—产地—形态分析联用的巴西草药DENTEDE LEO(蒲公英)的混伪品鉴定及生药学研究

The pharmacognosy research on the adulterants of Brazilian herb DENTE DE LEO based on a combined analysis of DNA barcoding-origin-morphology

目的对巴西市场上销售的药用蒲公英的混伪品进行鉴定及生药学研究。方法通过提取样品DNA、PCR扩增及双向测序,获得样品的ITS序列。采用相似度分析法进行分子鉴定;根据植物产地分布特征和形态特征验证结果,三种方法联用获得鉴定结果;分析混伪品的性状和显微特征。结果 DNA条形码研究表明巴西药用蒲公英的混伪品来源于菊科植物假蒲公英猫儿菊Hypochaeris radicata的全草,产地分析和形态分析验证了DNA条形码鉴定结果。结论巴西药用蒲公英的混伪品来源于菊科蒲公英属植物假蒲公英猫儿菊Hypochaeris radicata的全草。混伪品的生药学特征能够为巴西草药DENTE DE LEO的真伪鉴别提供依据。

Objective To study a new adulterant of DENTE DE LEO in Brazil and perform a pharmaceutical analysi. Methods Total genomic DNA was isolated from the materials,,and nuclear DNA ITS sequences were amplified and sequenced. Similarity identification of BLAST analysis was performed. Next,the results were verified according to plant geographical distribution and morphological profiles. Finally,the features of adulterant were demonstrated through microscopic observation. Results Adulterants of Brazil herbs DENTE DE LEO was Hypochaeris radicata Linnaeus,Sp. Pl. The origin analysis and morphological analysis verified the results of DNA barcoding. Conclusion Adulterants of Brazilian herb DENTE DE LEO are from the Asteraceae plant Hypochaeris radicata with the whole plants.Adulterants pharmacognosy features can provide the basis for identification of Brazil herbs DENTE DE LEO.