基于DNA条形码—产地—形态分析联用的巴西草药CENTELHA ASITICA(积雪草)的生药学研究

The pharmaceutical research on Brazilian herb CENTELHA ASITICA( Centella asiatica) based on a combined analysis of DNA barcoding-origin-morphology

目的对巴西草药CENTELHA ASITICA进行生药学研究。方法采用DNA条形码技术对巴西草药的叶片提取DNA、扩增ITS序列、双向测序,采用相似度法进行物种鉴定,再结合产地信息和性状特征核对DNA条形码鉴定结果进行验证。结果巴西草药CENTELHA ASITICA与NCBI核酸数据库积雪草ITS序列达到最高相似度,相似度为99%,鉴定其为伞形科植物积雪草Centella asiatica(L.)Urban。在地理分布上,巴西草药CENTELHA ASITICA符合积雪草的分布特征;从形态分析上,样品与积雪草特征相符合。结论基于DNA条形码-产地-形态分析的鉴定方法能够对巴西草药CENTELHA ASITICA进行鉴定,鉴定该物种来源于伞形科植物积雪草Centella asiatica(L.)Urban的干燥全草。生药学研究能够为CENTELHA ASITICA品种鉴定及质量标准研究提供依据。

Objective To study Brazilian herbal CENTELHA ASITICA from the perspective of biological pharmacy. Methods By applying DNA barcoding technology,total genomic DNA was isolated from the materials,and nuclear DNA ITS sequences were amplified and sequenced; DNA fragments were collated and matched by using Conting Express. Similarity identification of BLAST analysis was performed.Next,the DNA bar code was verified by the information of plant origin and morphology. Families and genera were identified by Molecular identification. Results ITS sequence of Brazil herb CENTELHA ASITICA and Centella asiatica（ L.） Urban was 99% identical. The results can be solidified by their similarities in geographical distribution and morphological features. Conclusion Based on DNA barcoding-origin-morphological analysis Brazilian herbal CENTELHA ASITICA was identified as Centella asiatica L. and biological pharmacy can provide the basis for the identification and quality standard of CENTELHA ASITICA varieties.