哈蟆油匀浆水溶液抗疲劳作用研究

Study on the anti fatigue effect of oviductus ranae homogenate solution

目的研究哈蟆油的抗疲劳作用。方法制备0.5%g/m L哈蟆油匀浆水溶液,采用小鼠灌胃给药,按成人日摄入量的1、10、30倍(即0.3、3.3、10.0 m L/kg BW)连续灌胃30天,与阴性对照组比较,测定负重小鼠游泳后血清尿素、肝糖原和血乳酸的含量。结果 10.0 m L/kg BW剂量组能延长小鼠负重游泳时间,减少小鼠游泳时血清尿素和血乳酸的产生,与阴性对照组比较均有显著性差异(P<0.05)。结论成人每天服用20 m L的0.5%g/m L哈蟆油匀浆水溶液(0.1 g哈蟆油)有明显的抗疲劳作用。

Objective To study the anti-fatigue effect of Oviductus Ranae. Methods The Oviductus Ranae homogenate solution 0. 5% g / m L was prepared,the mice were orally administered the drug into stomach at doses that 1,10 and 30 times the average adult amount of to adult（ 0. 3,3. 3,10 m L / kg BW） for 30 days,and then loaded them before determining the level of serum urea,liver glycogen and blood lactic acid. The results between intervention and control groups were then compared. Results Mice from 10 m L / kg BW dose group exhibited a prolonged the swimming time,and reduced blood urea nitrogen and blood lactic acid production levels.These differences were statistically significant between intervention the control groups（ P 0. 05）. Conclusion0. 5% g / m L of Oviductus Ranae homogenate solution serves anti-fatigue effects in mice.