莫诺苷对局灶性脑缺血再灌注大鼠血管内皮生长因子和成纤维生长因子-2表达的影响

Effects of Morroniside on Expression of Vascular Endothelial Growth Factor and Fibroblast Growth Factor-2 in Rats after Focal Cerebral Ischemia-reperfusion

目的观察莫诺苷对局灶性脑缺血再灌注大鼠皮层血管内皮生长因子(VEGF)和成纤维生长因子-2(FGF-2)表达的影响。方法 25只成年雄性Sprague-Dawley大鼠随机分为假手术组、模型组、莫诺苷小剂量组(30 mg/kg)、莫诺苷中剂量组(90 mg/kg)和莫诺苷大剂量组(270 mg/kg)。采用改良线栓法制备大鼠大脑中动脉栓塞(MCAO)30 min再灌注模型。再灌注7 d后利用蛋白免疫印迹法检测梗死侧皮层VEGF和FGF-2蛋白表达。结果与假手术组相比,缺血再灌注7 d后,各组梗死侧大脑皮层VEGF、FGF-2的表达均有增加(P<0.05);莫诺苷小、中、大剂量组VEGF表达进一步增加(P<0.05);莫诺苷大剂量组FGF-2的表达进一步显著增加(P<0.001)。结论莫诺苷可以促进局灶性脑缺血再灌注后VEGF和FGF-2的表达,可能有助于促进缺血性脑损伤后的血管新生。

Objective To investigate the effects of morroniside on the expression of vascular endothelial growth factor （VEGF） and fi-broblast growth factor-2 （FGF-2） in rat cortex after focal cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were ran-domly divided into sham group, model group, morroniside-low group （30 mg/kg）, morroniside-middle group （90 mg/kg） and morroni-side-high group （270 mg/kg）. Middle cerebral arteries of rats were occluded for 30 minutes with Longa＇s method and re-perfused. The ex-pression of VEGF and FGF-2 in the ischemic ipsilateral cortex was detected with Western blotting 7 days after reperfusion. Results The ex-pression of both VEGF and FGF-2 increased in the ischemic ipsilateral cortexin in all the ischemic groups compared with the sham group （P〈0.05）. The expression of VEGF further increased in a dose-dependent manner in all the morroniside groups compared with that of model group （P〈0.05）, and the expression of FGF-2 increased in the morroniside-high group （P〈0.001）. Conclusion Morroniside could increase the expression of VEGF and FGF-2 after ischemia-reperfusion, which might promote angiogenesis.