电针三阴交、阴陵泉对腓肠肌损伤大鼠损伤后修复及生长因子表达的影响

Effects of Electroacupuncture at Sanyinjiao and Yinlingquan Acupoints on Muscle Repair and Expression of Growth Factors in Gastrocnemius Muscle Damaged Rats

目的探讨电针治疗腓肠肌损伤的可能机制。方法 30只雄性Sprague-Dawley大鼠随机分为空白组(n=10)、模型组(n=10)和电针组(n=10)。模型组和电针组采用腓肠肌钝挫伤的方法制备模型,电针组电针三阴交、阴陵泉穴14 d。治疗结束后取腓肠肌组织行HE染色,免疫组化法检测大鼠腓肠肌组织、ELISA法检测大鼠血清碱性成纤维细胞生长因子(b FGF)和胰岛素样生长因子-1(IGF-1)的表达。结果与模型组相比,电针组损伤程度较轻,腓肠肌组织b FGF、IGF-1表达明显升高(P<0.01),血清含量升高(P<0.05)。结论电针可以促进腓肠肌损伤大鼠损伤后修复,其机制可能与上调修复因子b FGF和IGF-1的表达水平有关。

Objective To investigate the effects of electroacupuncture at Sanyinjiao （SP6） and Yinlingquan （SP9） on muscle repair in the gastrocnemius muscle damaged rats and its possible mechanism. Methods 30 male Sprague-Dawley rats were randomly divided into blank group （n=10）, model group （n=10） and electroacupuncture group （n=10）. The latter 2 groups were modeled with gastrocnemius muscle crush injury. The electroacupuncture group received electroacupuncture at Sanyinjiao and Yinlingquan for 14 days. Their gastrocnemius was observed with HE staining, and the expressions of basic fibroblast growth factor （bFGF） and insulin-like growth factor-1 （IGF-1） in gastroc-nemius tissues and the serum were determined with immunochemical assay and enzyme-linked immunosorbent assay （ELISA）. Results Compared with the model group, there was less damage in the electroacupuncture group, with more expression of bFGF and IGF-1 in tis-sues （P〈0.01） and serum （P〈0.05）. Conclusion Electroacupuncture can promote muscle repair after gastrocnemius muscle damage in rats, which may be related to the increase of the bFGF and IGF-1 expression in gastrocnemius tissue and serum.