TMV侵染后烟草中信号分子水杨酸和过氧化氢的变化

Study on variation of signal molecule SA and H_2O_2 in TMV inoculated tobacco

以两个抗病品种、一个感病品种为材料,利用抗体和定位染色对TMV侵染后烟草中的信号分子水杨酸和过氧化氢的时空变化进行检测。结果表明:接种TMV能够诱导水杨酸(SA)含量的上升,到接种后12h达到最大,且接种叶比同侧上部叶上升幅度要大,品种间存在差异;抗病品种接种TMV能够诱导过氧化氢的产生,随着时间延长过氧化氢从局部向外扩散,而感病品种和磷酸盐缓冲液(PBS)对照不能激发过氧化氢的产生。

Two disease-resistant and one susceptible tobacco varieties were used to determine variation of signal molecule SA and H2O2 in tobacco inoculated with TMV by the method of antibody and location staining. Results showed that inoculation with TMV could induce the rise of SA which reached maximum in 12 h. SA content in the inoculated leaves rose higher than that in the same side upper leaves, and such difference was found among different varieties. TMV inoculation could also induce the rise of H2O2 and facilitate H2O2 spread from inside to outside while neither susceptible variety nor control group treated with PBS could.