绿茶对血管性认知功能障碍患者氧化应激反应的影响

The Influence of green tea on oxidative stress response of patients with vascular cognitive impairment

目的探讨饮用绿茶对血管性认知功能障碍(VCI)患者体内氧化应激反应的影响。方法将入选的VCI患者依据饮用绿茶的习惯分为两组,即饮茶者组和非饮茶者组,采用简易精神状态检查表(MMSE)和蒙特利尔认识评估量表(Mo CA)检测认知功能,取肘静脉血测定丙二醛(MDA)、4-羟基壬烯醛(4-HNE)及8-羟化脱氧鸟苷(8-OHd G)的含量。结果所入选的VCI患者中,饮茶者组的MMSE评分(26.62±0.41)稍高于非饮茶者组(26.17±0.38),统计学分析显示两组差异无统计学意义(P>0.05)。但饮茶者组的Mo CA评分(23.29±0.61)高于非饮用绿茶者组(21.12±0.50),差异具有统计学意义(P<0.05)。饮茶者外周血的MDA含量(2.345±0.3697)低于非饮茶者(4.437±0.3710),以及饮茶者外周血4-HNE含量(4.919±0.9378)低于非饮茶者(8.660±0.7883),差异均具有统计学意义(P<0.01),而饮茶者8-OHd G浓度(1845±121.5)虽低于非饮茶者(2322±203.4),但差异无统计学意义(P>0.05)。结论常饮绿茶可能具有抑制VCI患者的氧化应激反应,改善认知功能的作用,但绿茶对抑制氧化性DNA损伤的作用较弱。

Objective To explore the influence of drinking green tea on oxidative stress response of patients with vascular cognitive impairment（VCI）. Methods The involved patients with vascular cognitive impairment were divided into two groups according to the habit of drinking green tea： drinking green tea group and non-drinking green tea group. The cognitive function was measured by Mini Mental State Examination （MMSE） and Montreal Cognitive Assessment （MoCA）. The oxidative stress response was evaluated by the level of malondialdehyde （MDA）, 4-hydroxynonenal（4-HNE） and 8-hydroxy-2 ~ -deoxyguanosine （8-OHdG） in elbow venous blood. Results Of all participants with VCI, the scores of MMSE between drinking green tea group （ 26.62±0.41 ） and non-drinking green tea group （26.17±0.38） had no significant difference （P〉0.05） ,while the scores of MoCA in drinking green tea group （23.29± 0.61 ）higher than that in non-drinking green tea group （21.12 ± 0.50） and they had significant difference （P〈0.05）.The levels of MDA in peripheral blood with drinking green tea habit （2.345 ±0.3697） lowered than those with non-drinking green tea habit（4.437±0.3710）, and 4-HNE in peripheral blood with drinking green tea habit（4.919±0.9378） lowered than those with non-drinking green tea habit （8.660±0.7883） ,there were significant differences（P〈0.01） .The plasma 8-OHdG concentration in patient with drinking green tea habit （1845±121.5） was lower than that with non-drinking green tea habit（2322±203.4）, but there were no significant difference（P〉0.05）. Conclusion To drinking green tea frequently could inhibit the oxidative stress response and enhance the cognitive function in patients with VCI. The inhibitory effect of green tea to oxidative DNA injury had undefined.