COMT基因多态性与颅脑创伤患者执行功能障碍相关性的研究

Correlation between COMT allele gene and executive dysfunction in patients with craniocerebral trauma

目的探讨儿茶酚胺氧位甲基转移酶(COMT)基因多态性与颅脑创伤患者执行功能障碍的相关性。方法选取河北联合大学神经外科颅脑创伤患者168例为病例组和134例健康体检人群为对照组。采集患者静脉血检测COMT基因多态性,应用钟表绘画测验(CDT)、威斯康星卡片测验系统(WCST)对患者进行执行功能评估。结果正常对照组和病例组COMT基因型及等位基因频率之间分布差异无统计学意义(P>0.05);不同教育程度、损伤部位、损伤类型及有无饮酒的颅脑创伤患者间COMT基因型和等位基因频率差异无统计学意义(P>0.05);不同病情程度颅脑创伤患者间COMT基因型差异有统计学意义(P>0.05),而等位基因频率差异无统计学意义(P>0.05)。WCST评测显示携带G/G基因型颅脑创伤患者的完成分类数(CC)分值低于携带GA+AA基因型患者;而持续性错误数(RPE)、完成第一个分类所需应答数(RF)分值高于携带GA+AA基因型颅脑创伤患者(P<0.05)。结论 COMT G/G基因型与颅脑创伤患者执行功能障碍有一定关系,可能是颅脑创伤患者执行功能损伤的风险。

Objective To explore the correlation between COMT allele gene and executive dysfunction in patientswithcraniocerebraltrauma.Methods 168casesofpatientswithcraniocerebraltrauma（fromneurosurgery department of the Affiliated Hospital , Hebei United University ） as case group and 134 cases of regular physical examination as control group.COMT gene polymorphism was detected with polymerase chain reaction （ PCR ） techeqich;the executive function was evaluated with the Clock Drawing Test （ CDT） and Wisconsin Card Sorting Test （ WCST） .Results There were no difference in COMT genotype and allele frequency between case group and control group（ P〉0.05） .There were no difference in COMT genotype and allele frequency among the craniocerebral trauma patients of different education level, injury site, injury type and drinking or not; There was difference in COMT genotype between different severity craniocerebral trauma patients（ P〈0.05） ,while no difference in allele frequency（ P〉0.05） .WCST results showed that the CC scores of Those who carry G/G allele were lower than those carry GA＋AA allele,while RPE scores and RF scores higher those carry GA＋AA allele.（ P〈0.05） .Conclusion The genotype G/G can increase the risk of injury of executive function impairment in patients with craniocerebral trauma.