3种硅酸盐矿物粉体对V79和A549细胞的遗传毒性

The genetic toxicity of three kinds of silicate mineral dusts to V79 and A549 cells

通过噻唑蓝(MTT)比色法、微核试验、单细胞凝胶电泳实验(SCGE),检测了3种硅酸盐矿物粉体(绢云母、石英、钠长石)对V79和A549细胞存活率、微核及DNA损伤的影响,比较和探讨了3种硅酸盐矿物粉体细胞遗传毒性的差异。研究结果表明,3种硅酸盐矿物粉体均不同程度抑制细胞增殖,诱发细胞染色体和DNA损伤,作用大小顺序均为绢云母>石英>钠长石,且对V79细胞的增殖抑制率和DNA损伤率均明显强于A549细胞,推测一定浓度范围内的硅酸盐矿物粉体对细胞遗传物质的稳定性可能存在影响,对人体可能具有遗传毒性。

In this study, the methyl thiazolyl tetrazolium （MTT） colorimetric assay, micronucleus test and sin- gle cell gel electrophoresis （SCGE） were used to detect the per-millage of micronucleus, the survival rate and the degree of DNA damage of three kinds of silicate mineral dusts to V79 and A549 cells which included sericite, quartz and albite. The results show that the three kinds of silicate mineral dusts have different degrees of inhibi- tion of cell proliferation, cell chromosome and DNA damage, with the effect from high to low being sericite 〉 quartz 〉 albite, and the cell proliferation inhibition rate and damage rate d I）NA on V79 cells is significantly stronger than those of A549 ceils. It is thus inferred that a certain concentration of silicate mineral dusts may af- fect the stability of the genetic material of cells and the genetic toxicity of the human body.