摘要
目的:研究并观察补肾利湿法对痛风性关节炎大鼠白介素17(IL-17)表达水平的影响,探讨补肾利湿法防治急性痛风性关节炎(Gouty Arthritis,GA)的作用机制。方法:将84只Wistar大鼠随机分为7组:空白组(BG)、模型组(MG)、中药对照组(CMCG)、西药对照组(WMCG)及中药复方低、中、高浓度组(CMLG、CMMG、CMHG),每组12只。采用MSU联合草酰钾诱导大鼠急性痛风性关节炎模型,应用ELISA法,分别检测各组大鼠血清IL-17的含量。结果:与空白组比较,造模各组大鼠血清IL-17的水平明显升高,差异有显著统计学意义(P<0.01);与模型组比较,西药对照组、中药对照组、中药复方低、中、高浓度各组IL-17水平明显降低,差异有显著统计学意义(P<0.01);西药对照组与中药复方中浓度组比较,IL-17含量差异不大(P>0.01)。结论:补肾利湿法处方可明显抑制急性痛风性关节炎大鼠IL-17的表达,对防治痛风性关节炎具有一定作用。
Objective： To observe the effect of Bushen Lishi Method on gouty arthritis on rat interleukin 17（ IL-17） expression level and to study the mechanism of Bushen Lishi Method in the prevention and treatment of acute gouty arthritis（ GA）. Methods： A total of 84 Wistar rats were randomly divided into 7 groups： blank group,model group,Chinese medicine control group,western medicine control group and Chinese medicine with low,medium and high dose groups,with 12 rats in each group. Using the model of uric acid sodium combined with potassium oxonate to induce acute gouty arthritis,and then use ELISA method to detect the content of serum IL-17 of rats. Results： Compared with the blank group,the levels of the serum IL-17 of model group rats were significantly increased,and there was a significant difference（ P〈0. 01）; compared with the model group,western medicine control group,Chinese medicine control group,Chinese herbal compound in low,high concentration,the level of IL-17 of which were significantly reduced,and there was a significant difference（ P〈0. 01）; western medicine control group compared with Chinese herbal compound in middle concentration,the IL-17 content of them had little difference（ P〉0. 01）. Conclusion： The prescription of Bushen Lishi Method can reduce the expression of IL-17 in rats with acute gouty arthritis significantly,which has a certain effect on the prevention and treatment of gouty arthritis.
出处
《世界中医药》
CAS
2015年第10期1574-1577,共4页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81173170)
黑龙江省普通高等学校青年学术骨干支持计划(编号:1254G055)
