中药丹参及复方丹参片17种有效成分抗氧化作用的均匀设计-高通量筛选研究

The Application of Uniform Design-High Throughput Screening on Antioxidant Research of 17 Components Radix Salviae Miltiorrhizae and Compound Danshen Tablets

目的:在前期研究的基础上,以丹参及复方制剂复方丹参片有效成分组合活性评价为切人点,通过已经建立的多种抗氧化微孔板实验方法,探讨多因素、多水平均匀设计-高通量筛选(uniform design-high throughput screening,UD-HTS)中药研究的新模式。方法:通过SpcctraMax M5连续光谱酶标测试仪选择具有药理活性的丹参有效成分11种(其中脂溶性成分4种,水溶性成分7种)及复方丹参片有效成分6种,通过均匀设计,进行各种成分及不同浓度之间相互配伍组合,以抗2,2-二苯基-1-苦肼基自由基(2,2-diphenyl-1-picrylhydrazyl,DPPH)氧化活性、邻二氮菲-Fe^(2+)氧化法、还原能力测定及抗脂质过氧化法,评价各种有效成分组合对药效的影响。结果:经过初筛和复筛,初步得到了丹参最佳水溶性配伍、水溶性+脂溶性配伍及优化组合配伍的组合样品。结论:丹参各有效成分不同浓度均匀设计配伍,结合抗氧化高通量筛选技术可适用于传统多因素、多水平组合特点的大规模药效筛选研究的新方法。

Objective：On the basis of the previous studies,and further evaluate and research the bioaetive components group of Radix Salviae Miltiorrhizae （RXM） and compound Danshen tablets by multifactor and multilevel uniform design-high throughput screening method,to find a new pattern to research traditional Chinese medicine. Methods：By using SpectraMax M5 continuous spectrum enzyme sign reflectoscope reflector, 11 of bioactive components which been confirmed with pharmacological activities of RXM and compound Danshen tablets （4 of water-solubility and 7 of liposolubility） and compound Danshen tablets （6） were recombined to be a new compatible group through uniform design. And the effects of the anti-oxidant microplate quantification experiment models were detected by anti-DPPH effect, eliminating ratio of hydroxy free radical, reduction ability determination, and lipid peroxidation were established. Results：After high-throughout screening and rescreening,better watersolubility,better water-solubility add liposolubility,and optimal combination compatibility of RXM were obtained. Conclusion ：The results indicated that effective component of RXM compatibility of different concentrations of uniform design, and with uniform design-high throughput screening of anti-oxidant models of microplate quantification referred to research bioactive component of compound recipe of traditional Chinese medicine.