局灶性脑缺血-再灌注大鼠Wnt3a蛋白表达的变化

Temporal Changes of Wnt3a Protein Level in Rats after Focal Cerebral Ischemia Reperfusion

目的:观察局灶性脑缺血-再灌注大鼠Wnt3a蛋白表达的动态变化。方法:取80只SD大鼠,采用改良的Zea Longa线栓法栓塞大脑中动脉,缺血30 min后再灌注(reperfusion)3、6、12、24 h,3、7、14、28 d,按随机数字法分组。假手术组除未栓塞大脑中动脉外,其余操作与模型组相同。免疫印迹法分别检测各组大鼠皮质Wnt3a蛋白表达情况。结果:与假手术组相比,Wnt3a的表达从缺血-再灌注后12 h开始升高,在24 h时达到最高峰,之后7 d内维持较高水平,但14 d后下降,直至28 d时表达仍高于假手术组。结论:局灶性脑缺血-再灌注后,大脑皮质区Wnt3a的表达随时间而动态变化,提示可能激活了Wnt3a参与的调节神经发生的信号通路。

Objective： To investigate the dynamic change of Wnt3a in rats after focal cerebral ischemia reperfusion. Methods： 80 rats were induced by occlusion of middle cerebral artery with suture embolus and ischemia for 30 minutes,then randomly assigned into different groups to receive reperfusion for 3,6,12,24 h,3,7,14,28 d. Sham operation group did not receive no suture embolus. The infarct side of the cortex of rats for each time group was harvested for the assessment of protein expression of activated Wnt3a using western blot staining. Results： Compared with the sham operation group （0.66±0.04）, Wnt3a was slightly increased after 3 hours of focal cerebral ischemia reperfusion, and then reached the peak at 24 hours （1.57± 0. 18） and maintained a higher level within 7 days, declined after 2 weeks,but remained higher than the level of the sham operation group at 4 weeks （0.68 ±0.04）. Conclusion： Wnt3a level of the cortex changed over time after reperfusion,suggesting that this signaling pathway may be activated in the regulation of neurogenesis.