人参皂苷Rg3微乳的处方优化及质量评价

Optimization of Formulation and Quality Evaluation of Ginsenoside Rg3 Microemulsion

目的优化人参皂苷Rg3微乳的处方,并对其质量进行评价。方法采用HPLC测定人参皂苷Rg3在油相、表面活性剂和助表面活性剂中的溶解度,通过伪三元相图和星点设计-效应面法优选处方。结果油酸乙酯、EL35-PEG400与水的最佳质量比为8.25∶12∶4.75∶75,平均粒径为60.50 nm,Zeta电位为-15.63 m V。微乳经4000 r·min-1离心10 min后无分层现象。结论该工艺方法简便、稳定可行,适用于人参皂苷Rg3微乳的制备。

Objective To optimize the formulation of ginsenoside Rg3 microemulsion preparation and to evaluate its quality. Methods The solubility of ginsenoside Rg3 in different oils, surfactants and co-surfactants was determined by HPLC method. The formulation was optimized by pseudo-ternary phase diagram and central composite design-response surface methodology. Results The optimized formulation was as follows： the proportion of ethyloleate, EL35, PEG400 and water was 8.25 ： 12 ： 4.75 ： 75, and the average particle diameter of ginsenoside Rg3 microemulsion was 60.50 nm with zeta potential of -15.63 inV. There was no any stratification after centrifuging at 4000 r .min-1 for 10 rain. Conclusion The process isfeasible, stable and applicable for the preparation of ginsenoside Rg3 microemulsion.