壬基酚对雄性小鼠生精能力及睾酮的影响

Effects of Nonylphenol on Spermatogenesis Function and Testosterone of Male Mice

[目的]研究壬基酚对雄性小鼠生精能力及血清性激素睾酮的影响。[方法]雄性昆明种小鼠40只,随机分为4组,每组10只,分别为壬基酚25、50、100 mg/kg剂量染毒组和溶剂对照组(玉米油)。各组隔日灌胃给予相应剂量的壬基酚溶液,连续35 d后处死小鼠取血,用酶联免疫法测定小鼠血清睾酮的水平。取左侧睾丸做组织病理切片,取双侧附睾制备精子悬液,并在光学显微镜下进行精子计数,计算活精子率和精子畸形率。[结果]35 d后,与对照组相比,100 mg/kg剂量染毒组小鼠睾丸组织病理学切片显示曲细精管萎缩,体重减轻幅度升高,睾丸重量下降,精子数量、精子活动度均降低,精子畸形率增高(均P<0.05)。染毒组小鼠血清睾酮含量、精子数量与壬基酚浓度存在明显剂量-反应关系(r=-0.98,r=-0.96;P<0.05)。[结论]壬基酚对雄性小鼠生殖系统有一定损害作用,高剂量的壬基酚能损伤小鼠的生精功能。

[ Objective ] To assess the effects of nonylphenol on spermatogenesis function and serum sex hormone testosterone of male mice. [ Methods ] Forty Kunming adult male mice were randomly divided into control group （corn oil）, low- dose nonylphenol group （25 mg/kg）, middle-dose nonylphenol group （50 mg/kg）, and high-dose nonylphenol group （100 mg/kg）, ten mice for each group. Corresponding administrations were given by gavage every other day for 35 days. Then the mice were neutralized and serum samples were collected to assess serum testosterone level by enzyme linked immunosorbent assay. Left testis was used for biopsy, sperm suspension was prepared by both cauda epididymidis for sperm count under optical microscopy to calculate sperm viability and sperm malformation rate. [ Results ] After 35 d, compared with the control group, the pathological sections of the 100mg/kg nonylphenol group presented seminiferous tubule atrophy, combined with increased weight loss, declined testicular weight, sperm quantity, sperm activity, and increased sperm malformation rate （all P 〈 0.05）. The serum testosterone levels and sperm counts had obvious dose-response relationships with the concentration of nonylphenol in the exposed mice （r=-0.98, r=-0.96; P〈0.05）. [ Conclusion ] Nonylphenol could damage the reproductive system of male mice to a certain extent, and high doses of nonylphenol could damage the spermatogesis function of mice.