GSK3β、p-GSK3β^(ser9)、p-GSK3β^(Tyr216)在宫颈癌组织中的表达及临床意义

Expression and clinical significance of GSK3β,p-GSK3β^(ser9) and p-GSK3β^(Tyr216) in cervical carcinoma

目的检测糖原合成酶激酶3β(GSK3β)、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达水平在宫颈癌组织中的表达情况及其与临床病理因素的相关性。方法收集2012年1月至2014年12月间第四军医大学西京医院妇产科收治的宫颈鳞癌病理标本80例,采用免疫组化SP法对宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达水平进行检测,并分析GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达与临床病理因素之间关系。结果宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达阳性率分别为18.8%、68.8%和12.5%。早期宫颈癌患者中GSK3β和p-GSK3β^(Tyr216)表达显著高于中晚期宫颈癌患者(25.5%vs.4.0%,χ~2=5.193,P=0.029;76.4%vs 52.0%,χ~2=4.749,P=0.039),早期宫颈癌患者中p-GSK3β^(ser9)表达显著低于中晚期宫颈癌患者(5.5%vs.28.0%,χ~2=7.988,P=0.009)。在宫颈癌组织中随着细胞学分级的降低,P-GSK3β^(ser9)阳性率也随之升高(7.4%、7.9%和33.3%,χ~2=7.329,P=0.031)。淋巴结转移阳性的宫颈癌患者中GSK3β和p-GSK3β^(Tyr216)表达均显著低于淋巴结转移阴性的宫颈癌患者(5.9%vs.28.3%,χ~2=6.427,P=0.018;52.9%vs.80.4%,χ~2=6.878,P=0.014),p-GSK3β^(ser9)在淋巴结转移阳性的宫颈癌患者中表达显著高于淋巴结转移阴性的宫颈癌患者(23.5%vs.4.3%,χ~2=6.577,P=0.015)。结论宫颈癌组织中GSK3β、p-GSK3β^(ser9)和p-GSK3β^(Tyr216)表达与宫颈癌临床分期、细胞学分级和淋巴结转移相关,提示GSK3β基因可能在宫颈癌组织发生发展中发挥重要作用。

Objective To investigate the expressions of glycogen synthase kinase 3β（ GSK3β） , p-GSK3βser9 and p-GSK3βTyr216 in cervical carcinoma and their correlation with clinical pathological factors.Methods By using immunohistochemistry, GSK3β, p-GSK3βser9 and p-GSK3βTyr216 protein expressions were detected in 80 cases of cervical carcinoma admitted in Xijing Hospital of Fourth Military Medical University during January 2012 to December 2014.The correlations between expressions of GSK3β, p-GSK3βser9 and p-GSK3βTyr216 and clinicopathological factors were analyzed.Results The positive expression rates of GSK3β, p-GSK3βser9 and p-GSK3βTyr216 in cervical carcinoma were 18.8%, 68.8% and 12.5%, respectively.The expressions of GSK3βand p-GSK3βTyr216 in early stage group were significantly higher than those in advanced stage group （25.5%vs 4.0%, χ2 =5.193,P=0.029;76.4% vs 52.0%,χ2 =4.749,P=0.039）, but the expression rate of p-GSK3βser9 was obviously lower （5.5%vs 28.0%,χ2 =7.988,P=0.009）.The positive expression rates of p-GSK3βser9 in well and moderately differentiated tumors were lower than those in poorly differentiated tumors （7.4%, 7.9%and 33.3%,χ2 =7.329, P=0.031）.The expressions of GSK3βand p-GSK3βTyr216 were significantly lower in patients with positive lymph node metastasis than in those with negative lymph node status （5.9%vs 28.3%,χ2 =6.427,P=0.018;52.9%vs 80.4%,χ2 =6.878, P=0.014）, but the expression of p-GSK3βser9 was higher in patients with lymph node metastases （23.5% vs 4.3%, χ2 =6.577,P=0.015） .Conclusion The expressions of GSK3β, p-GSK3βser9 and p-GSK3βTyr216 in cervical carcinoma are correlated with clinical TNM staging, histological grades and lymph node metastases, which suggests that GSK3βmay play a potential role in cervical tumor genesis.