TNF-α在小儿结肠息肉中的表达及临床意义

Expression and clinical significance of TNF-α in children with colon polyps

目的：探讨内源性肿瘤坏死因子α（ TNF-α）在小儿结肠息肉中的表达及临床意义。方法采集45例结肠息肉患儿（实验组）及36例正常结肠粘膜儿童（对照组）的血清，运用夹心法酶联免疫吸附试验（ ELISA）方法检测TNF-α在两组血清中的表达；免疫组化法检测TNF-α在两组患儿结肠组织中的表达，分析TNF-α的表达与结肠息肉的关系。结果 TNF-α在实验组血清中的表达高于对照组（t＝5．360，P＜0．05），在实验组结肠组织中表达高于对照组（χ2＝10．434，P＜0．05）；在实验组中，腺瘤性息肉中TNF-α的表达较幼年性息肉中无明显差异（P＞0．05）。结论 TNF-α在结肠息肉组织中的高表达，提示TNF-α在儿童结肠息肉的发生发展中可能起重要作用，有可能成为判断结肠息肉预后复发的标志物。

Objective To investigate the expression and clinical significance of endogenous tumor necrosis factor alpha （ TNF-α） in children with colonic polyps.Methods Serum of 45 cases （ experimental group） with colonic polyps and 36 cases （ control group） with normal colonic mucosa was detected with enzyme-linked immunosorbent assay （ ELISA ） to explore the expression of TNF-α. Immunohistochemical method was used to detect the expression of TNF-αin colonic tissue.The relationship between expression of TNF-αand colonic polyps was analyzed.Results The expression of TNF-αin serum was higher in the experimental group than in the control group （t=5.360, P〈0.05）, so was in colonic tissue （χ2 =10.434,P〈0.05）.In the experimental group the expression of TNF-αwas not significantly different between adenomatous polyp and juvenile polyp （P〉0.05）.Conclusion High expression of TNF-αin colon polyps suggests that TNF-αmay play an important role in the development and progression of colon polyps in children.It may be a marker for judging the prognosis of colon polyps.