人热敏瞬时受体通道1基因转染对兔角膜内皮细胞的影响

Effects of human thermal transient receptor channel 1 gene transfection on cultured rabbit corneal endothelial cellsin vitro

目的:探讨人热敏瞬时受体通道1基因转染对培养的兔角膜内皮细胞增殖能力的影响。方法:研究组为人热敏瞬时受体通道1基因通过脂质体介导的方法转染到体外培养的兔角膜内皮细胞中,采用MTT方法观察对细胞增殖的影响,免疫组织化学染色法和计算机图像分析系统检测对细胞增殖细胞核抗原(proliferation cell nucleus antigen,PCNA)表达的影响。结果:热敏瞬时受体通道1基因转染后内皮细胞增殖增加,实验组与对照组比较差异有统计学意义(t=3.01,P=0.013);实验组细胞PCNA表达明显增加,与对照组比较差异有统计学意义(t=3.21,P=0.007)。结论:人热敏瞬时受体通道1基因转染可以促进兔角膜内皮细胞增殖。

AIM：To explore the effects of human thermal transient receptor channel 1 gene transfection on corneal endothelial cell of rabbits.METHODS：Research group were dealt for thermal transient receptor channel 1 gene mediated by liposome transfection to rabbit corneal endothelial cells.MTT method was used to observe its influence on cell proliferation.Immunohistochemical staining and computer image analysis system were used to test the effects for proliferation cell nucleus antigen（PCNA）expression.RESULTS：Proliferation of corneal endothelial cell of rabbit was promoted after thermal transient receptor channel 1 gene transfected and the difference between experiment group and control group（t=3.01,P=0.013）.The expression of PCNA promoted after thermal transient receptor channel 1 gene transfected（t=3.21,P=0.007）compared with control group.CONCLUSION：The expression of PCNA in rabbit corneal endothelial cells can promote the proliferation of corneal endothelial cells of rabbits.