Livin及Caspase-3在眼附属器淋巴组织增生病变中的表达

Expression of Livin and Caspase-3 in ocular adnexal lymphoproliferative lesions

目的:探讨眼附属器淋巴组织增生性病变组织中Livin和Caspase-3的表达情况及临床意义。方法:收集1995-06/2015-06青岛大学附属医院眼科病理室石蜡包埋标本,采用免疫组化法检测40例眼部B细胞非霍奇金淋巴瘤(non-Hodgkin lymphoma,NHL)、10例反应性淋巴组织增生组织中Livin和Caspase-3的表达情况,以患者的年龄、性别、发病部位、病理类型分级作为眼附属器淋巴瘤的分类标准。结果:Livin及Caspase-3的表达与年龄、性别及发病部位无关,而与病理分级有关,Livin在淋巴瘤中的表达率显著高于反应性淋巴组织增生组织(P<0.05),且随着病理分级恶性程度的增加其表达逐渐上升(P<0.05);Caspase-3在淋巴瘤中的表达率则显著低于反应性淋巴组织增生(P<0.05),且随病理分级的增加其表达逐渐降低(P<0.05)。在NHL中,Livin和Caspase-3两者的表达呈负相关(r=-0.491,χ2=7.519,P<0.05)。结论:过量的Livin表达及Caspase-3的低表达可能与眼部淋巴瘤的发生有关,且在NHL中两者表现为负相关,联合检测两种蛋白对眼部B细胞淋巴瘤的临床表现及病理分型有重要意义。

AIM：To investigate the expression of Livin and Caspase-3proteins in ocular adnexal lymphoproliferative lesions and its clinical significance.METHODS：Patients suffering from B-cell non-Hodgkin lymphoma（NHL）（n=40） and reactive lymphoid tissue hyperplasia（RLH）（n =10） of ocular adnexal from June 1995 to June 2015 in Qingdao Affiliated Hospital were observed and the 50 paraffin sections were collected.RLH sections were selected to the control group.The patients＇ age,gender,pathogenic site and pathological types were selected as the classification criteria.The expression of Livin and Caspase-3 proteins were detected by immunohistochemistry and the positive expression rate between lymphomas and RLH was compared.Spearman rank correlation was used to estimate the relationbetween Livin and Caspase-3 in ocular adnexal lymphoma.RESULTS：The expression of Caspase-3 in B-cell NHL was lower than that in lymphadenosis（P〈0.05）.While the expression of Livin in NHL was higher（P〈0.05）.The two proteins had no relation with the age,pathogenic site or gender.But they were related to pathologic type.Livin was highly increased in plasmacytoma（PL） and diffused large B-cell lymphoma（DLBCL） compared with mucosa-associated lymphoid tissue（MALT） lymphoma（P〈0.05）.While Caspase-3 in PL and DLBCL were lower than that in MALT lymphoma（P〈0.05）.There was a negative correlation between Livin and Caspase-3 in MALT（r=-0.491 X2 =7.519,P〈0.05）.CONCLUSION：Over expression of Livin and low expression of Caspase-3 may play a significant role in the occurrence,development and different pathologic type of ocular lymphoma.The expression of Livin and Caspase-3 combined with each other in NHL.Combined examination of two proteins may be a valuable marker to predict the presence and differentiate the pathologic type of ocular adnexal lymphomas.