生物样品中胍丁胺的定量分析方法及评价

Summary of Quantitative Analysis and Correlation Evaluation of Agmatine Content in Biological Samples

胍丁胺是精氨酸脱羧基生成的内源性代谢产物,在自然界中广泛存在于植物、细菌及哺乳类动物的多种组织中。研究表明胍丁胺有着重要的生物学特性,如增强吗啡镇痛,降低吗啡耐受及躯体依赖,故具有良好的新药开发前景。准确而快速地测定生物体内的胍丁胺对于研究其生物学功能具有十分重要的意义。目前,已有多种测量方法用于胍丁胺的定量测定,如色谱法、质谱法、电泳法、免疫法。该文对胍丁胺含量测定方法的研究进展、适用范围及其优缺点作一综述,旨在为后期对胍丁胺的深入研究提供方法学的参考。

Agmatine,an endogenous amine synthesized from the decarboxylation of arginine,presents in plants,bacteria and several mammalian tissues. Agmatine has many physiological functions, such as enhancing morphine analgesia, reducing the symptoms of dependence and tolerance. It has a good prospect of application in new drug development. For a better understanding of its physiological functions, the exact and fast determination of agmatine in biological samples is necessary. A variety of measurement methods including chromatography, mass spectrometry, electrophoresis and immunoassays have been used for the determination of agmatine levels. In this article, the research progress on determination of agmatine in biological samples reviewed; the advantages and disadvantages of the different determination methods and their applications are also discussed, and hence to provide methodology reference for the analysis of agmatine.