杧果果实响应1-MCP处理的数字基因表达谱分析

Transcriptome Profiling of Ripening Mango Fruit Treated with 1-MCP Using Digital Gene Expression(DGE)Sequencing

以‘台农1号’杧果为试材,用1–甲基环丙烯(1-MCP,1μL·L-1)常温下处理12 h和未处理的果实为对照,通过数字基因表达谱(DGE)技术,寻找1-MCP处理对杧果贮藏影响的潜在关键基因。DGE数据分析结果表明,1-MCP处理与对照样品文库相比,共检测到7 350个差异表达基因。其功能主要涉及细胞壁代谢,激素调节,逆境胁迫应答,氧化损伤保护,能量代谢,蛋白质折叠,泛素化和蛋白酶体途径介导的细胞程序性死亡,成熟与衰老调控等。利用实时定量PCR(q RT-PCR)技术对DGE部分数据进行验证,检测了其中6个较有代表性的应答基因的差异表达。通过基因本体(Gene Ontology,GO)通路功能富集分析表明,1-MCP处理增加果实对逆境胁迫的抵抗能力,抑制物质和能量代谢,减少细胞内钙的流失并保护果实细胞。qRT-PCR技术数据支持RNA-Seq的检测结果。

1-methylcyclopropene（1-MCP）is usually applied to extend shelf life of mango（Mangifera indica Linn‘Tainong 1＇）fruits and reduce post-harvest deterioration. In order to explore the influence of 1-MCP on the fruit storage,the harvested Tainong 1 mango fruits were treated with 1-MCP（1 μL · L-1）under room temperature for 12 hours,fruits without 1-MCP treatment served as the control. Transcriptome profiling of mature mango fruits was conducted by digital gene expression（DGE）sequencing method. Seven thousand three hundred and fifty differentially expressed genes（DEGs）were identified between 1-MCP treated and control fruits. The DEGs involved in many pathways including cell wall metabolism,hormone regulation,stress and defense,oxidative damage protection,energy and metabolism,protein folding,programmed cell death mediated by the ubiquitination and proteosome pathways,as well as ripening and senescence processes. To validate the DGE data,six genes were selected for real-time quantitative PCR（q RT-PCR）analysis. Gene ontology（GO）term enrichment analysis of DEGs revealed that 1-MCP treatment could protect fruit cells by enhancing the stress resistance,inhibiting substance and energy metabolism and reducing the intracellular calcium loss. The q RT-PCR analysis confirmed the accuracy of DGE results.