特应性皮炎患儿外周血CD4^+T细胞miR-155的表达与疾病严重程度的相关性

Correlation of miR-155 in peripheral blood CD4^+T cells from children with atopic dermatitis with disease severity

目的:确定特应性皮炎(AD)患儿外周血CD4+T细胞中miR-155的表达与疾病严重程度的相关性。方法:采用实时荧光定量聚合酶链反应(Real-time-PCR)检测45例AD患儿和38例健康者对照外周血CD4+T细胞中miR-155的表达,ELISA法检测血清IL-5和IFN-γ表达,并进行SCORAD评分。结果:AD组miR-155的表达较对照组明显升高(P<0.05);AD患者组miR-155的表达与SCORAD评分、血清IL-5表达水平呈正相关(均P<0.05),与血清IFN-γ表达水平呈负相关(P<0.05)。结论:AD患儿外周血CD4+T细胞中miR-155表达与疾病严重程度正相关,可作为评定AD严重程度的一个指标。

Objective：To determine the correlation of miR-155 in the peripheral blood CD4＋T cells from children with atopic dermatitis （ AD） with disease severity. Methods：The expression levels of miR-155 of 45 children with AD and 38 healthy controls were detected by Real time-PCR. The levels of serum IL-5 and IFN-γ were tested by ELISA. SCORAD of the patients were assessed. Results：The expression level of miR-155 of children with AD was higher than that in healthy controls （ P〈0.05） . The expression level of miR-155 was positively correlated with the SCORAD and serum IL-5 level （ P〈0.05） and negatively correlated with serum IFN-γ （P〈0.05）. Conclusion： miR-155 is positively correlated with the disease severity in children with AD, which can be used for a marker of disease severity in AD.