电化学DNA生物传感器的指示方法及其临床研究进展

The Indicating Methods of DNA Electrochemical Sensor and Progress of Clinical Research

电化学DNA生物传感器是很有前景的核酸分析技术,由于它操作简便快速、信号灵敏,能与DNA生物芯片兼容等优点,受到了广泛的关注,成为当今生物学、医学领域的前沿性课题。它利用杂交原理及电化学标志物为杂交检测信号,既可以用标记法又可以用非标记法进行检测,不受颜色影响,可对痕量样品测定,仪器设备易于集成化和自动化且价格低廉,正朝向便携式商业化方向发展。实现电化学DNA生物传感器的准确检测,实验中所用到的指示方法发挥很大作用。该文着重阐述在DNA传感器工作中所运用的指示方法以及其在临床中的研究进展。

DNA electrochemical biosensor is a very promising nucleic acid analysis technology, which is a novel and developing technique combining biochemical, electrochemical, medical and electronic techniques with the advantages of being simple, reliable, cheap, sensitive, selective for genetic detection and convenience for integration and microminiaturization, and has been a hot topic in the field of biochemistry and medicine. DNA electrochemical biosensor are commonly employed to detect specific sequences of DNA. The transduc- tion strategies can be subdivided into two main categories,labeled and label-free approaches. At present, it is developing toward the direction of portable commercial devices. Here is to make a review of the the indicating methods of DNA biosensors and the research development in the clinical application.