妇血宁颗粒对气虚血瘀模型大鼠凝血功能的影响

Effect of Fuxuening Granules on Coagulation Functions of Rats Models with Qi Deficiency and Blood Stasis Syndrome

目的 :观察妇血宁颗粒对气虚血瘀模型大鼠凝血功能的影响。方法 :通过力竭游泳加皮下注射肾上腺素的方法复制气虚血瘀模型大鼠,设置空白对照组、气虚血瘀模型组、传统汤剂组及妇血宁颗粒大、中、小剂量组,每组10只。空白对照组、气虚血瘀模型组给予生理盐水,传统汤剂组给予妇血宁汤剂,妇血宁颗粒大、中、小剂量组给予相应剂量的妇血宁颗粒。给药7 d后,腹主动脉取血,测定凝血活酶时间(APTT)、凝血酶原时间(PT)、血浆纤维蛋白原(FIB)含量和血流变指标。结果:与模型组比较,妇血宁颗粒各剂量组大鼠活化部分APTT、PT、FIB、血浆黏度及180 s-1、30 s-1和5 s-1血液黏度均降低;妇血宁颗粒大、中、小剂量大鼠APTT、PT及30 s-1、5 s-1血液黏度与模型组比较,有显著性差异(P<0.05),FIB、血浆黏度和180 s-1血液黏度与模型组比较,无显著性差异(P>0.05)。结论:妇血宁颗粒可改善气虚血瘀模型大鼠的凝血三项指标和血液黏度,但对其血浆黏度影响不大,为妇血宁颗粒的研制提供了药理实验依据。

Objective：To observe the effect of Fuxuening granules on coagulation functions of rats models with qi deficiency and blood stasis syndrome. Methods：The rats models of Qi deficiency and Blood stasis were established by subcutaneous injection of epinephrine and exhaustive swimming. The rats Were divided into six groups：blank control group,model of Qi deficiency and blood stasis group,traditional decoction group,group of large-dose Fuxuening granules,group of medium-dose Fuxuening granules：, and, group of small-dose Fuxuening granules,with ten in each group. The control group and the madel.model group were given normal saline. The traditional decoction group was given traditional decoction.：The groups,,of large-dose, medium-dose and small-dose Fuxuening granules were given correspond dose of FuxuSning grallules. The rats were sampled blood from the abdominal aorta to evaluate the acated,： partial throtnboplastin time （APTF）, prothrombin time （PT）, fibrinogen level （FIB） and blood rheology indexes after 7 day s, i Results ： The APTY,PT,FIB,plasma viscosity（PV） and blood viscosity of 180 s-1,30 s-1 and 5s-1 of all Fuxuening granules groups were reduced. All Fuxuening granules groups produced significant effects on APTr,PT and 30 s-1,5 s-1 blood viscosity（P〈0.05）,but have no significant effects on FIB,PV and 180 s-1 blood viscosity（P〉0.05）. Conclusion：Fuxuening granules can improve three coagulations functions and blood viscosity of rats with Qi deficiency and blood stasis syndrome ,but has little effect on their plasma viscosity. This provides a pharma-cological basis for development of Fuxuening gran- ules.