摘要
提出了一种从肝素提取过程中产生的废蛋白中提取类肝素的工艺。以类肝素总单位数为评价指标,通过单因素实验和正交设计实验确定了吸附工艺最佳条件:温度为55℃,盐浓度为0.8 mol/L,p H为8.72。氧化工艺最佳条件为:加入双氧水的量与待氧化的类肝素粗品溶液体积比为2∶100,氧化时间为6 h。与传统的处理废蛋白的方式相比,有效地增加了其经济价值。
A kind of extraction technology of heparinoid from waste protein generated in the heparin extraction process is proposed. With the total unit number of heparinoid as the assessment index, the optimum conditions for the adsorption process are determined by single factor and orthogonal design experiments as follows ：55℃ of the temperature, 0. 8 mol/L of the salt concentration and 8.72of pH value. Besides that,the optimum conditions for the oxidation process are also shown as follows ：2：100 of the volume ratio of hydrogen peroxide and 6 hours of the reaction time. Compared to the traditional processing method for waste protein, the economic value is efficiently improved. It provides a good reference for the heparin production enterprises.
出处
《现代化工》
CAS
CSCD
北大核心
2015年第11期125-128,共4页
Modern Chemical Industry
关键词
类肝素
吸附
氧化
单因素
正交设计
heparinoid
adsorption
oxidation
single factor
orthogonal design
