子痫前期患者胎盘组织中Syncytin-2和MFSD2的表达

Expression of Syncytin-2 and MFSD2 in placenta tissues of patient with preeclampsia

目的:探讨Syncytin-2及其受体MFSD2在不同程度子痫前期和正常晚期妊娠胎盘组织中的表达情况。方法:选择2010年3月至2013年8月在广州医科大学附属第三医院住院分娩的60例孕妇作为研究对象,分为轻度子痫前期组、重度子痫前期组和正常晚期妊娠组,每组各20例。采用定量RT-PCR方法检测Syncytin-2 mRNA的转录水平,Western blotting法测定MFSD2蛋白表达强度。结果:3组Sycytin-2mRNA和MFSD2蛋白相对表达量比较,差异均有统计学意义(P<0.05),其中正常晚期妊娠组高于轻度子痫前期组和重度子痫前期组(P<0.05),而轻度子痫前期组与重度子痫前期组比较,差异无统计学意义(P>0.05)。结论:胎盘组织Syncytin-2和MFSD2表达下调与子痫前期发病密切相关。

Objective： To investigate the expression of Syncytin-2 and its receptor MFSD2 in placenta tissues of different levels of preeclampsia and normal late pregnancy. Methods： Sixty pregnant women hospitalized for childbirth in the Third Affiliated Hospital of Guangzhou Medical University between March 2010 and August 2013 were included as the subjects in the study, and were divided into the mild preeclampsia group, severe preeclampsia group,and normal late pregnancy group（ n = 20 each）. The transcription level of Syncytin-2mRNA was determined by quantitative real-time PCR（ RT-PCR）,and the expression intensity of MFSD2 protein was determined by Western blotting. Results： There were statistically significant differences in the relative expression levels between Syncytin-2 mRNA and MFSD2 protein among the three groups（ P〈0. 05）. The relative expression levels of Syncytin-2 mRNA and MFSD2 protein in the normal late pregnancy group was higher than those in the mild preeclampsia group and severe preeclampsia group（ P〈0. 05）,whereas no statistically significant differences was found between the mild preeclampsia group and severe preeclampsia group（ P〈0.05）. Conclusion： Down-regulated expressions of Syncytin-2 and MFSD2 in placenta tissue are closely related to the occurrence of preeclampsia.