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去细胞神经复合脂肪干细胞修复神经缺损的实验研究

Experimental study of acellular nerve and adipose derived stem cells in repairing of nerve defects
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摘要 目的:探讨去细胞同种异体神经复合脂肪干细胞(ADSC)诱导分化的类许旺细胞在修复大鼠坐骨神经缺损中的效果。方法健康F344近交系大鼠30只,随机分为2组,每组15只,分别用不同的移植物桥接10 mm坐骨神经缺损,A组用单纯去细胞同种异体神经(SD大鼠去细胞神经支架)桥接,B组用脂肪干细胞诱导分化的类许旺细胞复合去细胞同种异体神经桥接。比较两组术后6、12周坐骨神经功能指数恢复率(SFI%)、神经电生理(NEP)包括坐骨神经传导速度(MNCV)恢复率和小腿腓肠肌复合动作电位(CAMP)恢复率、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定、组织学光镜和透射电镜观察髓鞘再生情况等指标的变化,评价实验效果。结果术后6、12周,B组SFI%(6周时52.38±7.12,12周时79.99±10.33)、MNCV恢复率(6周时51.12±8.15,12周时75.93±5.95)、CAMP恢复率(6周时45.38±4.12,12周时75.98±10.99)、再生有髓神经纤维计数恢复率(6周时44.29±7.52,12周时77.06±11.54)、神经纤维直径恢复率(6周时43.58±4.87,12周时83.23±6.32)、髓鞘厚度恢复率(6周时46.41±4.35,12周时83.96±8.31)等指标均优于A组SFI%(6周时46.32±5.13,12周时72.73±8.06)、MNCV恢复率(6周时42.54±6.33,12周时69.34±9.13)、CAMP恢复率(6周时40.52±4.15,12周时68.24±9.45)、再生有髓神经纤维计数恢复率(6周时38.45±6.28,12周时67.89±11.87)、神经纤维直径恢复率(6周时40.11±4.32,12周时76.37±9.38)、髓鞘厚度恢复率(6周时41.33±4.58,12周时77.62±7.98)(均P〈0.05)。B组神经组织学光镜观察可见神经纤维直径、数量及髓鞘厚度优于A组,透射电镜观察可见B组神经组织较A组髓鞘排列致密,髓鞘较厚。结论脂肪干细胞诱导分化的类许旺细胞复合去细胞同种异体神经构建的组织工程化神经能有效地修复大鼠坐骨神经缺损。 Objective To investigate the efficacy of allogenic acellular nerve scaffold and adipose derived stem cells (ADSCs)-differentiated Schwann-like in repairing the sciatic nerve defect of rats. Methods Thirty healthy F344 inbred rats were randomly divided into two groups (n=15 each). In these groups,a 10-mm sciatic nerve defect was bridged with different grafts:allogenic acellular nerve(from SD rat)scaffold in Group A,and allogenic acellular nerve scaffold seeded with Schwann-like cells differentiated from ADSCs in Group B. After 6 and 12 weeks,the both groups were compared for recovery rate of sciatic functional index (SFI%) and the neural electrophysiology (NEP) which included recovery rates of motor nerve conduction velocity (MNCV) of sciatic nerve, compound muscle action potential (CAMP) of gastrocnemius,regenerated myelinated fiber counts,nerve fiber diameter and myelin sheath thickness,as well as histological changes with myelin regeneration under light microscopy and transmission electron microscopy,so as to evaluate the experimental efficacy. Results At 6 and 12 weeks after operation,the Group B appeared advantageous over Group A in SFI%(at 6 weeks,52.38 ± 7.12 vs 46.32 ± 5.13;at 12 weeks,79.99 ± 10.33 vs 72.73 ± 8.06),MNCV recovery rate(at 6 weeks,51.12 ± 8.15 vs 42.54 ± 6.33;at12 weeks,75.93 ± 5.95 vs 69.34 ± 9.13),CAMP recovery rate(at 6 weeks,45.38 ± 4.12 vs 40.52 ± 4.15;at 12 weeks,75.98 ± 10.99 vs 68.24 ± 9.45),regenerated myelinated nerve fiber count recovery rate(at 6 weeks, 44.29±7.52 vs 38.45±6.28;at 12 weeks,77.06±11.54 vs 67.89±11.87),nerve fiber diameter recovery rate (at 6 weeks,43.58 ± 4.87 vs 40.11 ± 4.32;at 12 weeks,83.23 ± 6.32 vs 76.37 ± 9.38),myelin sheath thickness recovery rate(at 6 weeks,46.41±4.35 vs 41.33±4.58;at 12 weeks,83.96±8.31 vs 77.62±7.98) (all P〈0.05). Light microscopy revealed that the diameter and number of nerve fiber and the myelin sheath thickness were more favorable in Group B than in Group A. Transmission electron microscopy revealed that the nerve tissue was more densely arranged and with thicker myelin in Group B than as found for Group A. Conclusion Tissue-engineered nerve constructed from ADSCs-differentiated Schwann-like cells combined with allogenic acellular nerve can effectively repair nerve defects in rats.
出处 《中华生物医学工程杂志》 CAS 2015年第4期291-295,共5页 Chinese Journal of Biomedical Engineering
基金 郑州大学附属肿瘤医院科技拔尖人才项目(201406)
关键词 脂肪干细胞 许旺细胞 神经再生 组织工程 Adipose derived stem cells Schwann cells Nerve regeneration Tissue engineering
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